Osthole ameliorates myonecrosis caused by Clostridium perfringens type A infection in mice  

作　　者：Xueyong Zhang Yue Liu Zhangqi Shen Shaolin Wang Congming Wu Dejun Liu Shusheng Tang Chongshan Dai 

机构地区：[1]National Key Laboratory of Veterinary Public Health and Safety,College of Veterinary Medicine,China Agricultural University,Beijing 100193,China [2]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China

出　　处：《One Health Advances》2023年第1期52-61,共10页全健康进展（英文）

基　　金：funded by Laboratory of Lingnan Modern Agriculture Project(Award number NT2021006);supported by the National Natural Science Foundation of China(Award number 32102724);Pinduoduo-China Agricultural University Research Fund(No.PC2023A01002).

摘　　要：This study aimed to investigate the protective effect of the nature product osthole(OST)against Clostridium perfrin-gens type A infection-caused myonecrosis in a mouse model.Male mice were divided into(1)control,(2)infected,(3)OST50 and(4)OST100 treatment groups.In the infected groups,mice were intramuscularly injected with 1×10^(8) CFU of C.perfringens per day for 6 days.Mice in the OST50 and OST100 groups were administrated intraperitoneally with OST at the doses of 50 or 100 mg/kg per day post C.perfringens infection.Our results showed that C.perfringens infection caused marked necrosis and inflammatory cell infiltration in the muscle tissues of mice.Mice in the OST50 and OST100 treatment groups displayed significantly attenuated C.perfringens infection-induced lipid peroxida-tion,oxidative stress,and apoptosis in their muscle tissue.Furthermore,OST treatment significantly downregulated the expressions of NF-κB,IL-1β,and TNF-αmRNA and protein levels,while concomitantly upregulating the expressions of Nrf2 and HO-1 mRNA and protein.OST treatments also inhibited the expression of phosphorylation(p)-p38,p-mTOR,and p-Erk1/2 proteins,and upregulated LC3II and Beclin1 proteins.In summary,our results reveal that OST therapy confers a protective effect against C.perfringens infection-induced oxidative stress and inflammation in muscle tissue,via activation of Nrf2/HO-1 and autophagy pathways and inhibition of p38,Erk1/2 and NF-κB pathways.

关 键 词：C.perfringens MYONECROSIS OSTHOLE Nrf2/HO-1 pathway NF-κB pathway 

分 类 号：R285[医药卫生—中药学]