结膜黑色素瘤组织中S100A4蛋白表达及其预后预测价值  

Expression of S100A4 protein in conjunctival melanoma and its prognostic value

作　　者：李红梅 孟佳[2] 郝燕燕[2] LI Hongmei;MENG Jia;HAO Yanyan(Department of Ophthalmology,Lingbao Fushoutang Ophthalmology Hospital,Sanmenxia 472500,China;Department of Ophthalmology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]灵宝福寿堂眼科医院眼科,河南三门峡472500 [2]郑州大学第一附属医院眼科,河南郑州450052

出　　处：《肿瘤基础与临床》2024年第4期373-377,共5页journal of basic and clinical oncology

基　　金：河南省科技攻关计划项目(242102310437)。

摘　　要：目的探究结膜黑色素瘤组织中S100A4蛋白表达及其预后预测价值。方法选取2021年1月至2023年1月于灵宝市福寿堂眼科医院、郑州大学第一附属医院接受手术治疗的56例结膜黑色素瘤患者为研究对象。采用免疫组化法S-P法检测S100A4蛋白在结膜黑色素瘤组织中的表达,根据检测结果将入组患者分为S100A4蛋白高表达组(n=35)和S100A4蛋白低表达组(n=21),比较S100A4表达与总生存时间的关系。依据结膜黑色素瘤患者的预后情况将入组患者分为预后良好组(n=18)、预后不良组(n=38),分析可能影响结膜黑色素瘤患者预后不良的危险因素,根据危险因素构建Logistic回归预测模型,采用受试者工作特征曲线评估该预测模型效能。结果结膜黑色素瘤患者中位总生存时间为10个月,术后1 a存活率为80.36%(45/56)。S100A4蛋白高表达组患者中位生存时间、术后1 a生存率(9个月,74.58%)均低于S100A4蛋白低表达组(10个月,90.47%;Log-rankχ^(2)=3.485,P=0.001)。预后不良组患者肿瘤低分化、淋巴结转移、S100A4蛋白高表达比例分别为68.42%、73.68%、71.05%,均高于预后良好组的22.22%、27.78%、22.22%(χ^(2)=10.481,P=0.001;χ^(2)=10.635,P=0.001;χ^(2)=11.785,P=0.001)。多因素Logistic回归分析结果显示,肿瘤低分化(OR=2.042,95%CI:1.350~2.734)、淋巴结转移(OR=1.876,95%CI:1.225~2.526)、S100A4蛋白高表达(OR=1.828,95%CI:1.354~2.301)是影响黑色素瘤患者预后的危险因素。S100A4蛋白表达Logistic回归预测模型预测结膜黑色素瘤患者预后的敏感性、准确性、阳性预测值、曲线下面积分别为92.16%、90.02%、89.34%、0.914。结论肿瘤低分化、淋巴结转移、S100A4蛋白高表达是影响结膜黑色素瘤患者预后不良的危险因素,基于上述因素构建的模型预测结膜黑色素瘤患者预后不良的效能良好。Objective To investigate the expression of S100A4 protein in conjunctival melanoma and its prognostic value.Methods Fifty-six patients with conjunctival melanoma who received surgical treatment in the Lingbao Fushoutang Ophthalmology Hospital and the First Affiliated Hospital of Zhengzhou University from January 2021 to January 2023 were selected as the study objects.Immunohistochemical SP method was used to detect the expression level of S100A4 protein in conjunctival melanoma tissue,according to the detection results,the enrolled patients were divided into S100A4 protein high expression group(n=35)and S100A4 protein low expression group(n=21),and the relationship between S100A4 expression level and overall survival time was compared.According to the prognosis of conjunctival melanoma patients,the enrolled patients were divided into good prognosis group(n=18)and poor prognosis group(n=38),and the risk factors that may affect the poor prognosis of conjunctival melanoma patients were analyzed,a Logistic regression prediction model was constructed according to risk factors,and receiver operating characteristic curve was used to evaluate the effectiveness of the prediction model.Results The median survival time and 1-year survival rate of patients with conjunctival melanoma were 10 months and 80.36%.The median survival time and 1-year survival rate(9 months,74.58%)of and patients with high expression of S100A4 protein in conjunctival melanoma tissue were lower than those of patients with low expression of S100A4 protein(10 months,90.47%;Log-rankχ^(2)=3.485,P=0.001).The rates of low tumor differentiation,lymph node metastasis and high S100A4 protein expression in the poor prognosis group(68.42%,73.68%,71.05%)were higher than those in the good prognosis group(22.22%,27.78%,22.22%;χ^(2)=10.481,P=0.001;χ^(2)=10.635,P=0.001;χ^(2)=11.785,P=0.001).Logistic multivariate regression analysis showed that tumor differentiation was low(OR=2.042,95%CI:1.350-2.734),lymph node metastasis(OR=1.876,95%CI:1.225-2.526)or S100A4 pro

关 键 词：结膜黑色素瘤 S100A4蛋白 预后 预测价值 

分 类 号：R739.7[医药卫生—肿瘤]