红景天苷对大鼠蛛网膜下腔出血早期脑损伤保护作用的研究  

Protective effect of salidroside on early brain injury in rats with subarachnoid hemorrhage

作　　者：王涛[1] 岳双柱[1] 李文超 李富光 周文科[1] WANG Tao;YUE Shuangzhu;LI Wenchao;LI Fuguang;ZHOU Wenke(Henan Key Laboratory of Nerve Repair,the First Affiliated Hospital of Xinxiang Medical College,Weihui 453100,Henan,China)

机构地区：[1]新乡医学院第一附属医院河南省神经修复重点实验室,河南卫辉453100

出　　处：《现代中西医结合杂志》2024年第14期1899-1904,2003,共7页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81541030);河南省重点研发与推广专项(科技攻关)(202102310708)。

摘　　要：目的探究红景天苷对大鼠蛛网膜下腔出血后脑组织水肿、炎症反应、神经元凋亡的影响及其机制。方法将144只雄性SD大鼠随机分为4组,每组36只。模型组及红景天苷组大鼠通过颈内动脉穿刺法构建蛛网膜下腔出血模型。模型构建成功后,红景天苷组大鼠给予红景天苷50 mg/(kg·d)腹腔注射,每24 h注射1次,空白组、假手术组大鼠于同时间点给予等量的生理盐水腹腔注射。分别于腹腔注射24 h、48 h、72 h后对各组大鼠进行改良Garcia评分及脑组织含水量测量;取腹腔注射72 h后大鼠脑组织,尼氏染色观察神经元结构形态,TUNEL染色观察神经元凋亡情况,RT-PCR法检测损伤脑组织中核因子E 2相关因子2(Nrf2)、血红素加氧酶-1(HO-1)mRNA表达情况,ELISA法测定损伤脑组织中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量。结果腹腔注射24 h、48 h、72 h后,模型组和红景天苷组改良Garcia评分均明显低于同时期空白组及假手术组(P均<0.05),脑组织含水量均明显高于同时期空白组及假手术组(P均<0.05);腹腔注射24 h、48 h后,模型组改良Garcia评分、脑组织含水量与红景天苷组比较差异均无统计学意义(P均>0.05);腹腔注射72 h后,红景天苷组改良Garcia评分明显高于模型组(P<0.05),脑组织含水量明显低于模型组(P<0.05)。尼氏染色显示,空白组及假手术组神经元规整,可见大量尼氏体;模型组神经元形态结构紊乱,尼氏体减少;红景天苷组神经元受损较轻,尼氏体较模型组增多。模型组脑组织中Nrf2、HO-1 mRNA相对表达量均明显低于空白组及假手术组(P均<0.05),红景天苷组脑组织中Nrf2、HO-1 mRNA相对表达量均明显高于模型组(P均<0.05)。假手术组、模型组、红景天苷组神经元凋亡指数和脑组织中IL-6、IL-1β、TNF-α含量均明显高于空白组(P均<0.05),红景天苷组神经元凋亡指数和脑组织中IL-6、IL-1β、TNF-αObjective It is to explore the effect and mechanism of salidroside on brain tissue edema,inflammatory response,and neuronal apoptosis in rats after subarachnoid hemorrhage.Methods One hundred and forty-four male SD rats were randomly divided into 4 groups,with 36 rats in each group.The rats in the model group and salidroside group were used to establish models of subarachnoid hemorrhage through internal carotid artery puncture.After the models were successfully established,the rats in the salidroside group were given salidroside 50 mg/(kg·d)intraperitoneally every 24 h,and the rats in the blank group and sham operation group were given an equal amount of saline intraperitoneally at the same time point.After 24 h,48 h and 72 h of intraperitoneal injection,the modified Garcia score and water content of brain tissue of the rats in each group were respectively evaluated or measured;the brain tissue of rats after 72 h of intraperitoneal injection was taken to observe the neuron structure and morphology by Nissen staining,the neuron apoptosis was observed by TUNEL staining,and the mRNA expressions of nuclear factor E 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)in the damage brain tissue were detected by RT-PCR,and the contents of interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the damaged brain tissues were detected by ELISA.Results After 24 h,48 h and 72 h of intraperitoneal injection,the modified Garcia scores of the model group and salidroside group were significantly lower,while the water content of brain tissue was significantly higher than those of the blank group and the sham operation group at the same period(all P<0.05);after 24 h and 48 h of intraperitoneal injection,the differences in the Garcia scores and water content of brain tissue were not statistically significant between the model group and salidroside group(all P>0.05);after 72 h of intraperitoneal injection,the modified Garcia score of the salidroside group was significantly higher,while the water content of

关 键 词：红景天苷 蛛网膜下腔出血 脑水肿 细胞凋亡 炎症反应 

分 类 号：R-332[医药卫生]