Guicao Baidu decoction in mitigating chemotherapy-induced myocardial injury:case report and mechanism investigation  

作　　者：Jia-Qi Fu Run-Jie Sun Man-Ya Yu Mu-Yun Wu Xing Cui 

机构地区：[1]The First School of Clinical Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250014,China [2]Center of Oncology and Hematology,The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250001,China

出　　处：《Natural Therapy Advances》2024年第3期29-36,共8页

基　　金：supported by the National Natural Science Foundation of China(No.82074348,No.82274491);the innovation and development joint project of Shandong Province Natural Science Foundation(ZR2023LZL009);the Jinan clinical medical science and technology innovation plan(No.202225014,202328072).

摘　　要：To observe the effects of GuiCaoBaiDu Decoction(GCBD)on chemotherapy especially doxorubicin(DOX)-induced myocardial cardiotoxicity(DIC)and explore the mechanisms.The present study presents a case demonstrating the preventive and therapeutic effects of GCBD on myocardial injury following chemotherapy.Then network pharmacology was employed to predict the targets of GCBD.Subsequently,a DOX-induced apoptosis model of H9C2 cardiomyocytes was established and co-cultured with serum containing GCBD serum.The viability and myocardial enzyme levels were evaluated using CCK8 assay and ELISA assay,TUNEL was using for apoptosis test.The GCBD effect was confirmed by tests of ROS andα-actinin levels,evaluation of mitochondrial morphology,and BAX co-localization with mitochondria.Furthermore,the expression levels of apoptosis-related molecules were determined via Western blotting.Additionally,a mouse model exhibiting DOX-induced cardiac functional impairment was generated and subsequently treated with GCBD.Myocardial enzyme level was tested at first,then echocardiography was tested,myocardial apoptosis in mice was observed through HE staining while related proteins were detected using IHC.Network pharmacological analyses revealed that GCBD exerts its effects on BAX,Caspase7,and other related molecules.Initially,we demonstrated the effective amelioration of DIC in cardiomyocyte viability,LDH/CK levels,α-actinin and ROS levels,and apoptosis by GCBD through improvements in TUNEL test,mitochondrial morphology and WB.The efficacy of GCBD in enhancing cardiac function in DIC mice has been validated through animal experiments.Taken together,our study showed that GCBD could significantly alleviate DOX induced myocardial injury by regulating mitochondrial apoptosis.The utilization of GCBD can effectively contribute to the prevention and treatment of chemotherapy-induced myocardial injury when anthracycline chemotherapy is employed in clinical practice.

关 键 词：Guicao Baidu decoction chemotherapy-induced myocardial injury case report ANGIOGENESIS 

分 类 号：R285[医药卫生—中药学]