Determination of total flavonoids content in buckwheat and inhibition of α-amylase activity  

作　　者：Huijing Chao Rong Yang Ming Yang Beijie Xu Xu Zhao 

机构地区：[1]Faculty of Functional Food and Wine,Shenyang Pharmaceutical University,Shenyang 110016,China

出　　处：《Journal of Polyphenols》2024年第3期98-105,共8页多酚杂志（英文）

摘　　要：Diabetes is one of the most difficult chronic diseases to cure in the world,which seriously affects people’s health and quality of life.Flavonoids in buckwheat can regulate blood glucose levels by inhibitingα-amylase activity.Therefore,sweet buckwheat produced in Inner Mongolia was used as the research object,and buckwheat fl avonoids were extracted by ultrasonic-assisted extraction method.Total fl avonoids content was determined by ultraviolet-visible spectrophotometry.With acarbose as the positive control,the inhibition test ofα-amylase was carried out by DNS colorimetry to study the inhibition behavior of fl avonoids onα-amylase activity.The results showed that the extraction process of flavonoids was stable and reliable,and the established method for the determination of flavonoids was simple,accurate and reproducible.The total flavonoids content of buckwheat samples was 2.706 mg/g,buckwheat total fl avonoids extraction solution had an inhibitory eff ect onα-amylase,and its median inhibition concentration(IC_(50))was 38.53 mg/mL.The results of this experiment provide a technical reference for the development and utilization of fl avonoids in Inner Mongolia sweet buckwheat,and provide a theoretical reference for the development and application of flavonoid-rich hypoglycemic food.

关 键 词：BUCKWHEAT total flavonoids content Α-AMYLASE activity inhibition 

分 类 号：R284[医药卫生—中药学] R587.1[医药卫生—中医学]