Seed amplification assay of nasal swab extracts for accurate and non-invasive molecular diagnosis of neurodegenerative diseases  

作　　者：Suying Duan Jing Yang Zheqing Cui Jiaqi Li Honglin Zheng Taiqi Zhao Yanpeng Yuan Yutao Liu Lu Zhao Yangyang Wang Haiyang Luo Yuming Xu 

机构地区：[1]Department of Neurology,The First Affiliated Hospital of Zhengzhou Uni-versity,Zhengzhou University,Zhengzhou,China [2]Department of Rhinology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou,China [3]The Academy of Medical Sciences of Zhengzhou University,Zhengzhou University,Zhengzhou,China [4]Henan Key Laboratory of Cerebrovascular Diseases,The First Affiliated Hospital of Zhengzhou University,Zhengzhou University,Zhengzhou,China [5]Institute of Neuroscience,Zhengzhou University,Zhengzhou,China

出　　处：《Translational Neurodegeneration》2023年第1期681-690,共10页转化神经变性病（英文）

基　　金：supported by the National Natural Science Foundation of China(U1904207 to Dr.Yuming Xu);the Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2020-PT310-01 to Dr.Yuming Xu).

摘　　要：Nasal swabs are non-invasive testing methods for detecting diseases by collecting samples from the nasal cavity or nasopharynx.Dysosmia is regarded as an early sign of coronavirus disease 2019(COVID-19),and nasal swabs are the gold standard for the detection.By nasal swabs,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acids can be cyclically amplified and detected using real-time reverse transcriptase-polymerase chain reaction after sampling.Similarly,olfactory dysfunction precedes the onset of typical clinical manifestations by several years in prion diseases and other neurodegenerative diseases,such as Alzheimer’s disease,Parkinson’s disease,dementia with Lewy bodies,and multiple system atrophy.In neurodegenerative diseases,nasal swab tests are currently being explored using seed amplification assay(SAA)of pathogenic misfolded proteins,such as prion,α-synuclein,and tau.These misfolded proteins can serve as templates for the conformational change of other copies from the native form into the same misfolded form in a prion-like manner.SAA for misfolded prion-like proteins from nasal swab extracts has been developed,conceptually analogous to PCR,showing high sensitivity and specificity for molecular diagnosis of degenerative diseases even in the prodromal stage.Cyclic amplification assay of nasal swab extracts is an attractive and feasible method for accurate and non-invasive detection of trace amount of pathogenic substances for screening and diagnosis of neurodegenerative disease.

关 键 词：Nasal swab Seed amplification assay Prion disease AMYLOID Neurodegenerative diseases 

分 类 号：R741[医药卫生—神经病学与精神病学]