Cross-species single-cell transcriptomic analysis of animal gastric antrum reveals intense porcine mucosal immunity  

作　　者：Xiaodan Wang Fan Hong Haonan Li Yalong Wang Mengxian Zhang Shibo Lin Hui Liang Hongwen Zhou Yuan Liu Ye‑Guang Chen 

机构地区：[1]The State Key Laboratory of Membrane Biology,Tsinghua‑Peking Center for Life Sciences,School of Life Sciences,Tsinghua University,Beijing 100084,China [2]Guangzhou Laboratory,Guangzhou 510005,China [3]Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China [4]The First Affiliated Hospital of Nanjing Medical University,300 Guangzhou Road,Nanjing 210029,Jiangsu,China [5]School of Basic Medicine,Jiangxi Medical College,Nanchang University,Nanchang 330031,China

出　　处：《Cell Regeneration》2023年第1期133-146,共14页细胞再生（英文）

基　　金：supported by grants from the National Key Research and Development Program of China(2017YFA0103601);the National Natural Science Foundation of China(31988101 and 31730056 to YGC).

摘　　要：As an important part of the stomach,gastric antrum secretes gastrin which can regulate acid secretion and gastric emptying.Although most cell types in the gastric antrum are identified,the comparison of cell composition and gene expression in the gastric antrum among different species are not explored.In this study,we collected antrum epithelial tissues from human,pig,rat and mouse for scRNA-seq and compared cell types and gene expression among species.In pig antral epithelium,we identified a novel cell cluster,which is marked by high expression of AQP5,F3,CLCA1 and RRAD.We also discovered that the porcine antral epithelium has stronger immune function than the other species.Further analysis revealed that this may be due to the insufficient function of porcine immune cells.Together,our results replenish the information of multiple species of gastric antral epithelium at the single cell level and provide resources for understanding the homeostasis maintenance and regeneration of gastric antrum epithelium.

关 键 词：Single cell RNA-seq Gastric antrum CROSS-SPECIES Porcine immunity 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]