SALL4、GS、HSP70在肝癌组织中的表达及其在早筛中的临床价值  

Expression of SALL4,GS and HSP70 in hepatocellular carcinoma tissues and its clinical value in early screening

作　　者：尹婷立 景建军 王滢 张迎 王银 YIN Tingli;JING Jianjun;WANG Ying;ZHANG Ying;WANG Yin(Department of Oncology,Santai County People's Hospital,Mianyang,Sichuan,China,621100)

机构地区：[1]三台县人民医院肿瘤科,四川绵阳621100

出　　处：《分子诊断与治疗杂志》2024年第8期1533-1536,共4页Journal of Molecular Diagnostics and Therapy

基　　金：四川省科技计划项目(2022KJ13)。

摘　　要：目的分析婆罗双树样基因4(SALL4)、谷氨酰胺合成酶(GS)、热休克蛋白70(HSP70)在肝癌组织中的表达及其在早筛中的临床价值。方法选取2020年7月至2023年6月三台县人民医院收治的153例原发性肝癌患者(肝癌组,Edmondson病理分级:Ⅰ~Ⅱ级58例,Ⅲ~Ⅳ级95例),以153例患者距肿瘤切缘>3 cm处癌旁组织作为对照组,以及同期良性肝病组织141例作为良性组。分析三组以及肝癌组不同分级患者SALL4蛋白、GS、HSP70表达情况,分析SALL4蛋白、GS、HSP70对肝癌早期诊断价值。结果SALL4蛋白、GS、HSP70在不同组织中阳性表达率均为:肝癌组>良性组>对照组,差异均有统计学意义(P<0.05);Ⅲ~Ⅳ级患者组织中SALL4蛋白、GS、HSP70表达阳性率均明显高于Ⅰ~Ⅱ级,差异均有统计学意义(P<0.05);依据ROC曲线可知,SALL4+GS+HSP70联合检测对肝癌早期诊断敏感度和特异度分别为95.10%、78.60%,AUC=0.838(95%CI:0.755~0.922),明显高于SALL4、GS、HSP70单独检测(P<0.05)。结论SALL4蛋白、GS、HSP70在肝癌组织中均为高表达,三者表达情况或可作为肝癌早期筛查的评估指标,在肝癌的早期诊断及病情评估中有重要的临床意义。Objective To analyze the expression of brachystegia⁃like gene 4(SALL4,SALL4),glutamine synthase(GS),heat shock protein 70(HSP70)in hepatocellular carcinoma tissues and their clinical value in early screening.Methods A total of 153 patients with primary liver cancer treated at Santai County People's Hospital from July 2020 to June 2023 were selected for the study.The liver cancer group included 58 cases of gradeⅠ~Ⅱand 95 cases of gradeⅢ~Ⅳbased on the Edmondson pathological grade.Additionally,153 cases of paracarcinoma tissues located more than 3 cm away from the tumor margin were used as the control group,and 141 cases of benign liver disease tissues were used as the benign group.The aim of the study was to analyze the expression of SALL4 protein,GS,and HSP70 in patients with different grades within the three groups,as well as in the hepatocellular carcinoma group.The goal was to determine the value of SALL4 protein,GS,and HSP70 for the early diagnosis of hepatocellular carcinoma.Results The positive expression rates of the SALL4 gene,GS,and HSP70 in different tissues were as follows:liver cancer group>benign group>control group.The difference was statistically significant(P<0.05).The positive rates of the SALL4 gene,GS,and HSP70 in gradeⅢ~Ⅳpatients were significantly higher than those in gradeⅠ~Ⅱ,with statistically significant differences(P<0.05).Based on the ROC curve,it is evident that the sensitivity and specificity of the SALL4+GS+HSP70 combined test for the early diagnosis of hepatocellular carcinoma were 95.10%and 78.60%,respectively,with an AUC of 0.838(95%CI:0.755⁃0.922).This was significantly higher than that of SALL4,GS,and HSP70 alone(P<0.05).Conclusions The SALL4 protein,GS,and HSP70 are highly expressed in liver cancer tissues.The expression of all three may be used as an assessment indicator for early screening of liver cancer.This has important clinical significance in the early diagnosis and disease assessment of liver cancer.

关 键 词：SALL4 GS HSP70 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]