Telomere‑to‑telomere and haplotype‑resolved genome of the kiwifruit Actinidia eriantha  

作　　者：Yingzhen Wang Minhui Dong Ying Wu Feng Zhang Wangmei Ren Yunzhi Lin Qinyao Chen Sijia Zhang Junyang Yue Yongsheng Liu 

机构地区：[1]School of Horticulture,Anhui Agricultural University,Hefei 230036,China [2]School of Forestry Science and Technology,Lishui Vocational and Technical College,Lishui 323000,China [3]Ministry of Education Key Laboratory for Bio‑Resource and Eco‑Environment,College of Life Science,State Key Laboratory of Hydraulics and Mountain River Engineering,Sichuan University,Chengdu 610064,China

出　　处：《Molecular Horticulture》2023年第1期361-371,共11页分子园艺（英文）

基　　金：Open access funding provided by Shanghai Jiao Tong University;supported by funds from the National Natural Science Foundation of China(31972474,31471157).

摘　　要：Actinidia eriantha is a characteristic fruit tree featuring with great potential for its abundant vitamin C and strong disease resistance.It has been used in a wide range of breeding programs and functional genomics studies.Previously published genome assemblies of A.eriantha are quite fragmented and not highly contiguous.Using multiple sequencing strategies,we get the haplotype-resolved and gap-free genomes of an elite breeding line“Midao 31”(MD),termed MDHAPA and MDHAPB.The new assemblies anchored to 29 pseudochromosome pairs with a length of 619.3 Mb and 611.7 Mb,as well as resolved 27 and 28 gap-close chromosomes in a telomere-to-telomere(T2T)manner.Based on the haplotype-resolved genome,we found that most alleles experienced purifying selection and coordinately expressed.Owing to the high continuity of assemblies,we defined the centromeric regions of A.eriantha,and identified the major repeating monomer,which is designated as Ae-CEN153.This resource lays a solid foundation for further functional genomics study and horticultural traits improvement in kiwifruit.

关 键 词：KIWIFRUIT Actinidia eriantha GENOME Telomere-to-telomere HAPLOTYPE Centromeric 

分 类 号：S663.4[农业科学—果树学]