烟酰胺核糖对MPP^(+)诱导SH-SY5Y细胞损伤的保护及机制研究  

Protective effects and mechanisms of nicotinamide riboside on MPP^(+)-induced injury in SH-SY5Y cells

作　　者：杨艳梅 隋易航 吕志宇 Yang Yan-mei;Sui Yi-hang;LüZhi-yu(Department of Neurology,Affiliated Hospital of Southwest Medical University,Luzhou,Sichuan 646000,China)

机构地区：[1]西南医科大学附属医院神经内科,四川泸州646000

出　　处：《中国现代医学杂志》2024年第17期61-66,共6页China Journal of Modern Medicine

基　　金：四川省科技计划课题(No:2021YFH0190)。

摘　　要：目的探究烟酰胺核糖(NR)对1-甲基-4-苯基-吡啶离子(MPP^(+))诱导的SH-SY5Y细胞损伤的保护作用及其潜在机制。方法取对数生长期的H-SY5Y细胞来完成实验,使用3 mmol/L MPP^(+)处理细胞构建帕金森病(PD)体外模型。细胞分为对照组(常规培养)、MPP^(+)组(MPP^(+)处理)、实验组(0.5 mmol/L NR和MPP^(+)处理)。细胞培养后检测细胞存活率、凋亡率,氧化应激水平[活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)],炎症水平[白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)],烟酰胺腺嘌呤二核苷酸(NAD^(+))水平等指标。结果与对照组比较,MPP^(+)组细胞凋亡率、ROS、MDA、TNF-α、IL-6水平均升高(P<0.05),细胞存活率、SOD、NAD^(+)水平均降低(P<0.05);与MPP^(+)组比较,实验组细胞凋亡率、ROS、MDA、TNF-α、IL-6水平均降低(P<0.05),细胞存活率、SOD、NAD^(+)水平均升高(P<0.05)。结论NR可以减轻MPP^(+)诱导的神经细胞损伤,其机制可能与补充NAD^(+)减轻细胞氧化应激和炎症反应有关。Objective To explore the protective effect and mechanism of nicotinamide riboside(NR)on SH-SY5Y cells damage induced by MPP^(+).Methods SH-SY5Y cells in the logarithmic growth stage were used in the experiment.Cells were treated with 3 mmol/L MPP^(+)to establish Parkinson disease(PD)model.The cells were divided into control group(cells were cultured normally),MPP^(+)group(cells treated with MPP^(+)),NR group(cells treated with MPP^(+)and 0.5 mmol/L NR).After culturing,cell survival rate,apoptosis rate,oxidative stress level[reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD)],inflammation level[interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)]and nicotinamide adenine dinucleotide(NAD^(+))levels were detected.Results Compared with the control group,the cell apoptosis,ROS,MDA content,TNF-α,and IL-6 were increased,while the cell survival rate,SOD activity and NAD^(+)levels were decreased(P<0.05)in the MPP^(+)group.Compared with the MPP^(+)group,the ROS,MDA content,TNF-α,and IL-6 levels were significantly decreased,while the cell survival rate,SOD activity and NAD^(+)levels were significantly increased(P<0.05)in the NR group.Conclusion NR attenuates MPP^(+)-induced injury in neural cells,which may be involved in oxidative stress and inflammation through NAD^(+) supplementation.

关 键 词：帕金森病 烟酰胺核糖 NAD^(+) 氧化应激 

分 类 号：R741[医药卫生—神经病学与精神病学]