QuEChERS-GC-MS法快速检测茶叶中的芳樟醇和alpha-紫罗兰酮  

A Rapid QuEChERS-GC-MS Assay for Linalool and Alpha-ionone Determinations in Tea

作　　者：游佩琼 YOU Pei-qiong(Fujian Inspection and Research Institute for Product Quality,Fuzhou,Fujian 350002,China)

机构地区：[1]福建省产品质量检验研究院,福建福州350002

出　　处：《茶叶学报》2024年第3期57-66,共10页Acta Tea Sinica

摘　　要：【目的】为完善茶叶中香气物质的检测方法,针对芳樟醇与alpha-紫罗兰酮两种香气物质建立气相色谱-质谱联用法(Gas Chromatography-Mass Spectrometry,GC-MS)快速同时检测的分析方法,以期为茶叶中香气物质的检测提供新思路和参考。【方法】茶叶样品经乙腈提取后,用QuEChERS净化管(内含PSA 200 mg、GCB 200 mg、无水硫酸钠400 mg)净化茶叶上清液,采用GC-MS选择离子监测模式进行测定,外标法定量。【结果】芳樟醇和alpha-紫罗兰酮在5.0~2000.0μg·L^(−1)内线性良好,线性相关系数(R^(2))均大于0.999;方法的检出限为分别为0.020 mg·kg^(−1)和0.004 mg·kg^(−1),定量限分别为0.080 mg·kg^(−1)和0.010 mg·kg^(−1)。使用该方法在空白茶叶样品中分别添加3种不同加标浓度(5.0、10.0、1000.0μg·L^(−1))的混合标准溶液,测得平均回收率为88.23%~106.11%,相对标准偏差(RSD)为1.98%~3.25%(n=6)。【结论】该方法操作快速便捷,运行时间仅需16.8 min,重现性和精确度高、灵敏度好,适用于茶叶中芳樟醇、alpha-紫罗兰酮的快速检测,能够为相关机构对茶叶中香气物质的检测提供技术支持。【Objective】An assay to detect the aromatic linalool and alpha-ionone in tea applying gas chromatographymass spectrometry(GC-MS)was developed.【Method】Tea samples were firstly extracted by acetonitrile to collect the supernatant for the QuEChERS purification on a column containing 200 mg of PSA,200 mg of CB,and 400 mg of anhydrous sodium sulfate prior to GC-MS analysis.Under the ion monitoring mode(SIM),aromatic compounds were quantifiably determined with external standards.【Result】The assay detection limit on linalool was 0.020 mg·kg^(−1)and that on alpha-ionone 0.004 mg·kg^(−1);while the quantification limit was 0.080 mg·kg^(−1)on linalool and 0.010 mg·kg^(−1)on alpha-ionone.The measurement curves on the two aromatherapy substances were linear in concentrations of 5.0-2000.0μg·L^(−1)with a greater than 0.999 correlation coefficient(R^(2)).On the blank control sample of tea spiked with 5.0,10.0,and 1000.0μg·L^(−1)of standard linalool and alpha-ionone,the assay showed an average recovery of 88.23%-106.11%with a relative standard deviation(RSD)of 1.98%-3.25%(n=6).【Conclusion】The newly developed method was rapid with a running time of only 16.8 m and highly reproducible,accurate,and sensitive results.It was deemed applicable for rapid detection of linalool and alpha-ionone in tea.

关 键 词：茶叶 香气物质 QUECHERS GC-MS 芳樟醇 alpha-紫罗兰酮 

分 类 号：S571.1[农业科学—茶叶生产加工]