13份姜荷花品种花粉形态观察  

Observation on Pollen Morphology of 13 Curcuma alismaifolia Varieties

作　　者：石丁蓉 冷青云[2,3] 谌振[2,3] 黄素荣[2,3] 奚良 张琼尹 王健[1] 杨光穗[2,3] Shi Dingrong;Leng Qingyun;Shen Zhen;Huang Surong;Xi Liang;Zhang Qiongyin;Wang Jian;Yang Guangsui(College of Horticulture,Hainan University,Haikou,570208;Hainan Province Tropical Ornamental Plant Germplasm Innovative Utilization Engineering Technology Research Center,Institute of Tropical Crops Variety Resources,Chinese Academy of Tropical Agricultural Sciences,Danzhou,571737;Key Laboratory of Crop Genetic Resources and Germplasm Creation(Ministry of Agriculture and Rural Affairs),Institute of Tropical Crop Variety Resources,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;Analysis and Testing Center,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)

机构地区：[1]海南大学园艺学院,海口570208 [2]中国热带农业科学院热带作物品种资源研究所,海南省热带观赏植物种质创新利用工程技术研究中心,儋州571737 [3]中国热带农业科学院热带作物品种资源研究所,农业农村部华南作物基因资源与种质创制重点实验室,海口571101 [4]中国热带农业科学院分析测试中心,海口571101

出　　处：《分子植物育种》2024年第17期5769-5776,共8页Molecular Plant Breeding

基　　金：海南省重点研发项目(ZDYF2023XDNY069)资助。

摘　　要：为了明确姜荷花不同品种花粉的形态特征及各品种之间的亲缘关系,为姜荷花品种的鉴定和利用提供重要的参考,本研究以13个姜荷花品种的花粉为试材,通过场发射环境扫描电镜对花粉形状、花粉大小及花粉外壁纹饰等形态特征进行观察,并进行比较分析。研究结果表明:姜荷花花粉存在形式为单粒,无萌发孔,花粉表面具有小颗粒状的乌氏体及黏性蛋白,花粉粒外部形态呈长球形或近球形。不同品种的花粉形状、花粉大小、花粉外壁褶皱程度均存在差异。极轴长(38.84±1.44)μm~(65.36±5.92)μm,赤道轴长(35.83±3.95)μm~(51.20±7.11)μm,花粉大小(P×E)为(1 412.12±108.22)μm~2~(3 355.71±591.01)μm~2,皱纹宽度为(0.29±0.05)μm~(0.73±0.14)μm。花粉外壁纹饰为脑皱纹,花粉大小等级为中大等。根据极轴(P)、赤道轴(E)、P/E、P×E、脑皱纹宽共5个定量特征进行系统聚类分析。13个品种姜荷花在欧式距离为15时可分成4个类群,根据聚类结果与DNA倍性大小进行育性相关分析,本研究可大致反映不同姜荷花品种间的亲缘关系,为姜荷花花粉形态的育性、分类、亲缘关系和演化提供孢粉学依据。In order to cl arify the morphological characteristics of different varieties of Curcuma alismaifolia flowers and the relationship between various varieties,provide important references for the identification and utilization of Curcuma alismaifolia varieties,this study uses 13 Curcuma alismaifolia varieties as a trial material,and the environment is launched through the field to launch the environment.Scan the electron microscope to observe the shape of pollen,the size of the pollen and the outer wall of the pollen,and analyze it.The research results show that the existence of Curcuma alismaifolia pollen is single-grained,no cute hair holes,and the surface of the pollen has small granules and glutinous proteins.The external shape of the pollen grains is long or near-ball.There are differences in the shape of the pollen,the size of the pollen,and the outer wall folds of the pollen.The axis length is(38.84±1.44)μm~(65.36±5.92)μm,the equatorial shaft length is(35.83±3.95)μm~(51.20±7.11)μm,the size of the pollen(P×E)is(1412.12±108.22)μm2~(3355.71±591.01)μm2,the width width is(0.29±0.05)μm~(0.73±0.14)μm.The outer wall of the pollen is brain wrinkles,and the pollen size grade is medium and large.According to the axis(P),equatorial axis(E),P/E,P×E,and brain wrinkle width,a total of 5 fixed quantitative features are performed.13 varieties of Curcuma alismaifolia can be divided into 4 categories when the distance between European style is 15.According to the category results and DNA double-scale analysis,this study can roughly reflect the relationship between different Curcuma alismaifolia varieties,and provide palynological basis for the fertility,classification,relationship and evolution of pollen morphology of Curcuma alismaifolia.

关 键 词：姜荷花 花粉形态 扫描电镜 倍性鉴定 

分 类 号：S682.29[农业科学—观赏园艺]