基于柱前衍生化-GC-MS技术探究不同采摘期青钱柳叶挥发性物质组成  

Study on the Composition of Volatile Substances in Leaves of Cyclocarya paliurus at different Picking Stages by Pre-column Derivatization and GC-MS Technology

作　　者：汪安 程桂林 苏敏 查萍萍 汪生 WANG An;CHENG Gui-lin;SU Min(Chizhou Quality Supervision and Inspection Research Institute,Chizhou,Anhui 247099)

机构地区：[1]池州市质量监督检验研究院,安徽池州247099 [2]安徽医科大学科研实验中心,安徽合肥230032

出　　处：《安徽农业科学》2024年第17期189-193,共5页Journal of Anhui Agricultural Sciences

基　　金：安徽省药品监督管理局2022年度科技创新项目(AHYJ-KJ-202217)。

摘　　要：[目的]采用GC-MS技术,解析青钱柳叶中的挥发性物质组成,探究13批次不同采摘期青钱柳叶中挥发性物质含量差异。[方法]用甲醇超声、甲醇加热回流、乙醇超声、乙醇加热回流、乙酸乙酯超声和正己烷超声6种提取方法,分别对青钱柳叶的挥发性成分进行提取,再结合BSTFA衍生法,综合探究青钱柳叶的挥发性成分组成;研究2023年4月15日—11月1日13个不同采摘期样品中的挥发性物质含量差异。[结果]乙酸乙酯提取-BSTFA衍生化法能更全面探究青钱柳叶中挥发性物质的组成,研究共鉴定出25种物质,青钱柳叶中挥发性物质主要是亚麻酸、棕榈酸、呋喃果糖、丙三醇、叶绿醇和硬脂酸等,脂肪酸总含量约为50%,还包括小分子有机酸、醇类和单糖类物质等。S1~S9和S10~S13批次样本物质的含量存在一定的差异。[结论]春夏季采摘的青钱柳叶不饱和脂肪酸含量较高,秋季单糖类物质含量明显增高。[Objective]GC-MS technology was used to analyse the composition of volatile substances in the leaves of Cyclocarya paliurus,and to explore the differences in the content of volatile substance in 13 batches of Cyclocarya paliurus leaves at different picking periods.[Method]The volatile components of Cyclocarya paliurus leaves were extracted by methanol ultrasound,methanol heating reflux,ethanol ultrasound,ethanol heating reflux,ethyl acetate ultrasound and n-hexane ultrasound extraction methods,respectively,and then combined with BSTFA derivatization method to comprehensively explore the volatile composition of Cyclocarya paliurus leaves.The difference of volatile substances content in 13 batches of Cyclocarya paliurus leaves samples in different picking periods from April 15,2023 to November 1,2023 was studied.[Result]The ethyl acetate ultrasound extraction and BSTFA derivatization method could comprehensively explore the composition of volatile substances in the leaves of Cyclocarya paliurus.A total of 25 substances were identified in the study.The volatile substances in the leaves of Cyclocarya paliurus mainly included linolenic acid,palmitic acid,fructofuran,glycerol,phytol and stearic acid.The total content of fatty acids was about 50%,the small molecule organic acids,alcohols and monosaccharides were also included.There was a certain difference in the content of substances in the samples between S1-S9 and S10-S13 samples.[Conclusion]Unsaturated fatty acids were higher in Cyclocarya paliurus leaves picked in spring and summer,while the monosaccharides were significantly higher in autumn.

关 键 词：青钱柳叶 挥发性物质 采摘期 柱前衍生化-GC-MS 

分 类 号：R284[医药卫生—中药学]