Rosuvastatin‑Eluting Gold‑Nanoparticle‑Loaded Perivascular Wrap for Enhanced Arteriovenous Fistula Maturation in a Murine Model  

作　　者：Carleigh Klusman Benjamin Martin Joy Vanessa D.Perez Allan John R.Barcena Marvin R.Bernardino Erin Marie D.San Valentin Jossana A.Damasco Huckie C.Del Mundo Karem A.Court Biana Godin Gino Martin Canlas Natalie Fowlkes Richard Bouchard Jizhong Cheng Steven Y.Huang Marites P.Melancon 

机构地区：[1]Department of Interventional Radiology,The University of Texas MD Anderson Cancer Center,Houston 77030,USA [2]Baylor College of Medicine,Houston 77030,USA [3]College of Medicine,University of the Philippines Manila,1000 Manila,Philippines [4]Department of Nanomedicine,Houston Methodist Research Institute,Houston 77030,USA [5]Department of Chemistry,Lamar University,Beaumont 77710,USA [6]Department of Veterinary Medicine and Surgery,The University of Texas MD Anderson Cancer Center,Houston 77030,USA [7]Department of Imaging Physics,The University of Texas MD Anderson Cancer Center,Houston 77030,USA [8]Section of Nephrology,Department of Medicine,Selzman Institute for Kidney Health,Baylor College of Medicine,Houston 77030,USA [9]The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences,Houston 77030,USA

出　　处：《Advanced Fiber Materials》2023年第6期1986-2001,共16页先进纤维材料（英文）

基　　金：the National Institutes of Health—National Heart,Lung,and Blood Institute(5HL141831-05 and 1R01HL159960-01A1);a Radiological Society of North America Research Seed Grant(RSD2012);a Society of Interventional Radiology Pilot Research Grant,an MD Anderson Center for Advanced Biomedical Imaging Pilot Project Program Research Grant;the National Institutes of Health—National Cancer Institute through MD Anderson's Cancer Center Support Grant(P30CA016672,used the Research Animal Support Facility and Small Animal Imaging Facility).

摘　　要：Arteriovenous fistulas(AVFs)are a vital form of AV access for patients requiring hemodialysis,but they link to overall morbidity and mortality when they fail to mature.The most common cause of AVF non-maturation is neointimal hyperplasia(NIH).To minimize the deleterious effects of NIH,a perivascular wrap composed of polycaprolactone(PCL),rosuvastatin(ROSU),and gold nanoparticles(AUNPs)was constructed.This study assessed the impact of ROSU-eluting,radiopaque resorbable perivascular wraps on pathologic NIH in a chronic kidney disease(CKD)rodent model of AVF.Electrospun PCL wraps containing AuNPs and/or ROSU were monitored for in vitro tensile strength,AuNP release,ROSU elution,and effect on cellular viability.The wraps were then implanted around an AVF in a CKD rodent model for in vivo ultrasound(US)and micro-computed tomography(mCT)imaging.AVF specimens were collected for histological analyses.Cell viability was preserved in the presence of both AuNP-and ROSU-containing wraps.In vitro release of ROSU and AuNPs correlated with in vivo findings of decreasing radiopacity on mCT over time.AuNP-loaded wraps had higher radiopacity(1270.0-1412.0 HU at week 2)compared with other wraps(103.5-456.0 HU),which decreased over time.The addition of ROSU decreased US and histologic measurements of NIH.The reduced NIH seen with ROSU-loaded perivascular wraps suggests a synergistic effect between mechanical support and anti-hyperplasia medication.Furthermore,AuNP loading increased wrap radiopacity.Together,our results show that AuNP-and ROSU-loaded PCL wraps induce AVF maturation and suppress NIH while facilitating optimal implanted device visualization.

关 键 词：Arteriovenous fistula Chronic kidney disease Nanoparticles POLYCAPROLACTONE RADIOPACITY ROSUVASTATIN 

分 类 号：TB383[一般工业技术—材料科学与工程]