聚乙二醇6000模拟干旱胁迫下药物浸种对2种绿肥种子萌发的影响  

Effect of Seeding Macrotyloma uniflorum and Vicia sativa Plants with Different Agents on Seed Germination under PEG-6000 Stress

作　　者：武瑞瑞[1] 程金焕[1] 李亚男[1] 娄予强[1] 何红艳[1] 杨旸[1] WU Ruirui;CHENG Jinhuan;LI Ya’nan;LOU Yuqiang;HE Hongyan;YANG Yang(Institute of Tropical and Subtropical Cash Crops,Yunnan Academy of Agricultural Sciences,Baoshan,Yunnan 678000,China)

机构地区：[1]云南省农业科学院热带亚热带经济作物研究所,云南保山678000

出　　处：《热带农业科学》2024年第6期48-54,共7页Chinese Journal of Tropical Agriculture

基　　金：国家重点研发计划项目(No.2022YFF130240403);保山市科技计划项目(No.2022zc06,No.2022zc03)。

摘　　要：为寻找在干旱胁迫下促进硬皮豆和箭舌豌豆萌发的最佳浸种药物及浓度。以箭舌豌豆和硬皮豆种子为试验材料,浓度2%、5%和10%聚乙二醇6000(PEG-6000)模拟干旱处理,用壳聚糖、水杨酸、氯化钙和乙烯利对种子进行引发处理,蒸馏水浸种作对照,测量发芽率、发芽指数、根鲜重、根长、根体积和根表面积等指标。结果表明,PEG-6000浓度为2%时,硬皮豆和箭舌豌豆萌发效果较好;PEG-6000浓度为5%和10%时,硬皮豆和箭舌豌豆种子萌发受抑制。2%PEG-6000溶液培养下,箭舌豌豆用药物引发后,测量指标随药物浓度升高而降低,分别在壳聚糖、水杨酸、氯化钙和乙烯利浓度为50、50、500、700 mg/L时达到最高值;硬皮豆在水杨酸50、200 mg/L和氯化钙1000 mg/L处理无萌发现象;在壳聚糖和乙烯利处理下,呈现先降低后升高变化趋势。用隶属函数法对绿肥种子发芽过程的6个生理生化指标进行综合评价,发现药物引发可缓解轻微干旱胁迫对种子萌发的影响,药物浸种24 h时,促进硬皮豆和箭舌豌豆种子萌发最佳处理方法分别是1 400 mg/L的乙烯利溶液和浓度为50 mg/L的壳聚糖溶液。This study aimed to identify optimal seed-dipping drugs and concentrations to promote the germination of M.uniflorum and V.sativa under drought stress.Seeds of M.uniflorum and V.sativa were used as test materials.Drought treat-ment was simulated with concentrations of 2%,5%,and 10%polyethylene glycol 6000(PEG-6000),and the seeds were treated with chitosan,salicylic acid,calcium chloride,and vinblastine.The seeds were soaked in distilled water as a control,and the germination rate,germination index,root fresh weight,root length,root volume,and root surface area were measured.The results of the study revealed that the germination of M.uniflorum and V.sativa was more than that at 2%PEG-6000,and the germination of M.uniflorum and V.sativa seeds was inhibited at 5%and 10%PEG-6000.The measured indices of V.sa-tiva treated with drugs in a 2%PEG-6000 solution decreased with increasing drug concentration.The germination indices de-creased with increasing concentrations of chitosan,salicylic acid,and calcium chloride,and the highest values were reached at concentrations of 50,50,500,and 700 mg·L^(-1) respectively.M.uniflorum peas did not germinate in the 50,200 mg·L^(-1) salicylic acid and 1,000 mg·L^(-1) calcium chloride treatments and tended to decrease but then increased in the chitosan and vin-blastine treatments.The six indices of the germination process of the green manure seeds were evaluated comprehensively according to their affiliated functions.Drug triggering could alleviate the effects of slight drought stress on seed germination,and drug-impregnated seeds were used to promote germination in the M.uniflorum and V.sativa seeds.The best treatments for promoting the germination of M.uniflorum and V.sativa seeds at 24 h were 1400 mg·L^(-1) ethylene glycol solution and 50 mg·L^(-1) chitosan solution,respectively.

关 键 词：硬皮豆 箭舌豌豆 聚乙二醇6000 药物浸种 隶属函数法 

分 类 号：S142[农业科学—肥料学]