LINC01410促进食管鳞状细胞癌细胞增殖和迁移的作用及其机制研究  

Mechanism of LINC01410 promoting proliferation and migration in esophageal squamous cell carcinoma

作　　者：曹飞[1] 俞文豪 唐晓男 马子冬 常廷民[3] 龚亚斌[4] 廖明娟[5] 康小红[2] CAO Fei;YU Wenhao;TANG Xiaonan;MA Zidong;CHANG Tingmin;GONG Yabin;LIAO Mingjuan;KANG Xiaohong(Department of Science and Education,Pingdingshan First People’s Hospital,Pingdingshan 467000,Henan Province,China;Department of Oncology,The First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,Henan Province,China;Department of Gastroenterology,The First Affiliated Hospital of Xinxiang Medical University,Xinxiang 453100,Henan Province,China;Department of Oncology,Yueyang Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Department of Traditional Chinese Medicine,Shanghai Ninth People’s Hospital of Shanghai Jiao Tong University School of Medicine,Shanghai 200125,China)

机构地区：[1]河南省平顶山市第一人民医院科教科,河南平顶山467000 [2]新乡医学院第一附属医院肿瘤科,河南新乡453100 [3]新乡医学院第一附属医院消化科,河南新乡453100 [4]上海中医药大学附属岳阳中西医结合医院肿瘤科,上海200437 [5]上海交通大学医学院附属第九人民医院中医科,上海200125

出　　处：《中国癌症杂志》2024年第8期753-762,共10页China Oncology

摘　　要：背景与目的:LINC01410是长度为647 bp的长链非编码RNA(long non-coding RNA,lncRNA),其异常表达参与多种肿瘤的发生、发展,但其对食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)的影响研究较少。本研究旨在探讨LINC01410促进ESCC细胞增殖和迁移的作用机制,以期为防治ESCC提供生物标志物及潜在治疗靶点。方法:应用基因表达谱交互分析2(Gene Expression Profiling Interactive Analysis 2,GEPIA2)在线分析LINC01410在肿瘤与癌症基因组图谱(The Cancer Genome Atlas,TCGA)ESCC数据中的表达水平及与患者预后总生存期的关系;采用基因探针富集分析(Gene Set Enrichment Analysis,GSEA)LINC01410可能调控的信号转导通路。收集2020年1月—2021年12月在新乡医学院第一附属医院、平顶山市第一人民医院胸外科行根治性手术的ESCC患者新鲜肿瘤组织标本62例,并选取癌旁组织作为对照。本项目经医院伦理委员会批准(新乡医学院第一附属医院,编号:2018036;平顶山市第一人民医院,编号:2019-018)。本研究采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)检测LINC01410的表达水平并分析其与临床病理学参数的关系。应用LV-NC、LV-over/LINC01410转染EC109细胞并建立稳定过表达细胞株EC109/NC、EC109/OE,采用shRNA-NC、shRNA-LINC01410转染EC9706细胞并建立稳定敲降细胞株EC9706/NC、EC9706/KD;应用RTFQ-PCR检测LINC01410的相对表达量;采用噻唑蓝比色法[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide,MTT]实验和克隆形成实验检测细胞增殖;采用transwell实验检测细胞迁移能力;采用双荧光素酶报告基因实验检测LINC01410对T细胞因子/淋巴增强因子1(T cell factor/lymphoid enhancer factor 1,TCF/LEF1)启动子活性的影响;采用蛋白质印迹法(Western blot)检测Wnt/β-连环蛋白(β-catenin)信号转导通路、上皮-间质转化(epithelial-mesenchymal transition,EMT)信号�Background and purpose:Long non-coding RNA(lncRNA)LINC01410,with a length of 647 bp,participates in a variety of tumor biological processes.However,the role and mechanism of LINC01410 involved in esophageal squamous cell carcinoma(ESCC)remain unclear.This study aimed to explore the potential mechanism of LINC01410 promoting ESCC proliferation and invasion,to provide a potential prognostic indicator and therapeutic target for individuals with ESCC.Methods:Gene Expression Profiling Interactive Analysis 2(GEPIA2)databases were used to analyze the expression of LINC01410 and overall survival in esophageal squamous cell carcinoma data set in the Cancer Genome Atlas(TCGA).Gene Set Enrichment Analysis(GSEA)was performed to identify the underlying signaling pathways involved in the biological effects of LINC01410 in ESCC.A total of 62 pairs of ESCC tissues and paracancerous tissues from ESCC patients who underwent radical surgery in the Department of Thoracic Surgery at the First Affiliated Hospital of Xinxiang Medical College and the First People's Hospital of Pingdingshan City from January 2020 to December 2021 were collected.This project has been approved by the Hospital Ethics Committee(First Affiliated Hospital of Xinxiang Medical College,No.2018036;First People's Hospital of Pingdingshan City,No.2019-018).The expression of LINC01410 in ESCC tissues was detected by real-time fluorescence quantitative polymerase chain reaction(RTFQ-PCR).We transfected EC109 cells with LV-NC or LV-over/LINC01410 and EC9706 cells with shRNA-NC or shRNA-LINC01410.Stable transfected cells(EC109/NC,EC109/OE,EC9706/NC and EC9706/KD)were selected in primary cell culture medium containing puromycin.The expression of LINC01410 was detected by RTFQ-PCR.The impact of LINC01410 on ESCC cell proliferation was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)and colony formation assays.The effect of LINC01410 on ESCC cell invasion was detected by transwell migration assay.T cell factor/lymphoid enhancer factor 1(TCF/L

关 键 词：食管鳞癌 长链非编码RNA-LINC01410 增殖 迁移 WNT/Β-CATENIN信号转导通路 

分 类 号：R735.1[医药卫生—肿瘤]