CSPP1 stabilizes microtubules by capping both plus and minus ends  

作　　者：Zhikai Wang Wenwen Wang Shuaiyu Liu Fengrui Yang Xu Liu Shasha Hua Lijuan Zhu Aoqing Xu Donald LHill Dongmei Wang Kai Jiang Jennifer Lippincott-Schwartz Xing Liu Xuebiao Yao 

机构地区：[1]MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics,Center for Advanced Interdisciplinary Science and Biomedicine of IHM,University of Science and Technology of China School of Life Sciences,Hefei 230027,China [2]Anhui Key Laboratory for Cellular Dynamics and Chemical Biology,Hefei National Research Center for Interdisciplinary Sciences at the Microscale,Hefei 230027,China [3]Frontier Science Center for Immunology and Metabolism,Medical Research Institute,Wuhan University,Wuhan 430071,China [4]Comprehensive Cancer Center,University of Alabama,Birmingham,AL 35233,USA [5]Janelia Research Campus,Howard Hughes Medical Institute,Ashburn,VA 20147,USA

出　　处：《Journal of Molecular Cell Biology》2024年第2期36-46,共11页分子细胞生物学报（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China and the National Natural Science Foundation of China(2022YFA1303100,32090040,92254302,2022YFA0806800,91854203,31621002,2017YFA0503600,21922706,and 92153302 to Xing Liu,2022YFA1302700 to Z.W.,32100612 to Xu Liu);the Ministry of Education of China(IRT_17R102,20113402130010,and YD2070006001 to Xing Liu);the Plans for Major Provincial Science&Technology Projects of Anhui Province(202303a0702003 to Xing Liu);the Fundamental Research Funds for the Central Universities(KB9100000007 and KB9100000013 to Xing Liu);University of Science and Technology of China Start-up Fund(KY9990000167 to Z.W.);Zhejiang Provincial Natural Science Foundation(LY23C070002 to W.W.)。

摘　　要：Although the dynamic instability of microtubules(MTs)is fundamental to many cellular functions,quiescent MTs with unattached free distal ends are commonly present and play important roles in various events to power cellular dynamics.However,how these free MT tips are stabilized remains poorly understood.Here,we report that centrosome and spindle pole protein 1(CSPP1)caps and stabilizes both plus and minus ends of static MTs.Real-time imaging of laser-ablated MTs in live cells showed deposition of CSPP1 at the newly generated MT ends,whose dynamic instability was concomitantly suppressed.Consistently,MT ends in CSPP1-overexpressing cells were hyper-stabilized,while those in CSPP1-depleted cells were much more dynamic.This CSPP1-elicited stabilization of MTs was demonstrated to be achieved by suppressing intrinsic MT catastrophe and restricting polymerization.Importantly,CSPP1-bound MTs were resistant to mitotic centromere-associated kinesin-mediated depolymerization.These findings delineate a previously uncharacterized CSPP1 activity that integrates MT end capping to orchestrate quiescent MTs.

关 键 词：CSPP1 MICROTUBULE plus end minus end capping 

分 类 号：Q24[生物学—细胞生物学]