Layer-by-layer assembly of procyanidin and collagen promotes mesenchymal stem cell proliferation and osteogenic differentiation in vitro and in vivo  

作　　者：Zhibiao Bai Kai Hu Zeyu Shou Jiahuan Yu Hongming Meng Han Zhou Liangyan Chen Tiantian Yu Ruofei Lu Na Li Chun Chen 

机构地区：[1]Department of Orthopaedics,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000,P.R.China [2]Wenzhou Medical University,Wenzhou 325000,P.R.China [3]Wenzhou Key Laboratory of Perioperative Medicine,Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine,Vision and Brain Health),Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou 325001,P.R.China [4]Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Urumqi 830011,P.R.China

出　　处：《Regenerative Biomaterials》2023年第1期333-343,共11页再生生物材料（英文版）

基　　金：supported by the National Key Research&Development Program of China(2019YFE0113600);the National Natural Science Foundation of China(NSFC 81971734,32071323,32271410);the Science and Technology Projects in Fujian Province(2022FX1,2023Y4008);the Program for Innovative Research Team in Science,Scientific Research Funds of Huaqiao University(21BS113)is gratefully acknowledged。

摘　　要：Collagen,commonly used in tissue engineering,is widespread in various tissues.During bone tissue regeneration,collagen can stimulate the cellular response and determine the fate of cells.In this work,we integrated collagen type II with procyanidin(PC)onto an implant coating by applying a layer-by-layer technique to demonstrate that collagen and PC can participate in the construction of new biomaterials and serve as multifunctional components.The effects of PC/collagen multilayers on the viability of cocultured bone marrow mesenchymal stem cells(BMSCs)were analyzed by cell counting kit-8 analysis and phalloidin staining.The reactive oxygen species level of BMSCs was revealed through immunofluorescent staining and flow cytometry.Osteogenesis-related genes were detected,and in vivo experiment was performed to reveal the effect of newly designed material on the osteogenic differentiation of BMSCs.Our data demonstrated that in BMSCs PC/collagen multilayers accelerated the proliferation and osteogenic differentiation through Wnt/β-catenin signaling pathway and enhanced bone generation around the implant in the bone defect model of rabbit femurs.In summary,combination of collagen and PC provided a new sight for the research and development of implant materials or coatings in the future.

关 键 词：bone marrow mesenchymal stem cells OSTEOGENESIS COLLAGEN procyanidine coating 

分 类 号：R318[医药卫生—生物医学工程]