Quercetin-solid lipid nanoparticle-embedded hyaluronic acid functionalized hydrogel for immunomodulation to promote bone reconstruction  

作　　者：Pinghui Zhou Bomin Yan Bangguo Wei Liangmin Fu Ying Wang Wenrui Wang Li Zhang Yingji Mao 

机构地区：[1]Department of Orthopaedics,The First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,China [2]School of Life Science,Bengbu Medical College,Bengbu 233030,China [3]Anhui Province Key Laboratory of Tissue Transplantation,Bengbu Medical College,Bengbu 233030,China [4]Department of Plastic Surgery,The First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,China [5]Anhui Province Key Laboratory of Translational Cancer Research,Bengbu Medical University,Anhui 233030,China

出　　处：《Regenerative Biomaterials》2023年第1期713-728,共16页再生生物材料（英文版）

基　　金：supported by grants from the 512 Talents Development Project of Bengbu Medical College(by51201206,by51202302 and by51202309);the Domestic Visiting and Training Program for Outstanding Young Backbone Teachers in High Schools(gxgnfx2022036);the Natural Science Research Project of the Anhui Educational Committee(KJ2021ZD0089 and 2022AH020086);the Scientific Research Foundation of Bengbu Medical College(2021bypd006);Nature Science Research Project of Anhui Province(2108085MH294).

摘　　要：Bone defects are a persistent challenge in clinical practice.Although repair therapies based on tissue-engineered materials,which are known to have a crucial role in defective bone regeneration,have gathered increased attention,the current treatments for massive bone defects have several limitations.In the present study,based on the immunomodulatory inflammatory microenvironment properties of quercetin,we encapsulated quercetin-solid lipid nanoparticles(SLNs)in a hydrogel.Temperature-responsive poly(ε-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone-co-lactide)modifications were coupled to the main chain of hyaluronic acid hydrogel,constructing a novel,injectable bone immunomodulatory hydrogel scaffold.Extensive in vitro and in vivo data showed that this bone immunomodulatory scaffold forms an anti-inflammatory microenvironment by decreasing M1 polarization,while elevating the M2 polarization.Synergistic effects on angiogenesis and anti-osteoclastic differentiation were observed.These findings further proved that administering quercetin SLNs encapsulated in a hydrogel can aid bone defect reconstruction in rats,providing new insights for large-scale bone defect repair.

关 键 词：QUERCETIN HYDROGELS MACROPHAGES inflammatory microenvironments bone tissue engineering 

分 类 号：R318.08[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]