Shear stress regulation of nanoparticle uptake in vascular endothelial cells  

作　　者：Hongping Zhang Ziqiu Hu Jinxuan Wang Jianxiong Xu Xiangxiu Wang Guangchao Zang Juhui Qiu Guixue Wang 

机构地区：[1]Key Laboratory for Biorheological Science and Technology of Ministry of Education,State and Local Joint Engineering Laboratory for Vascular Implants,Bioengineering College of Chongqing University,Chongqing 400030,China [2]Lab Teaching&Management Center,Chongqing Medical University,Chongqing 400016,China [3]JinFeng Laboratory,Chongqing 401329,China

出　　处：《Regenerative Biomaterials》2023年第1期1048-1059,共12页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(12032007,31971242)to G.W.;the Chongqing Science and Technology Bureau(cstc2019jcyj-zdxmX0028)to G.W.;JinFeng Laboratory,Chongqing,China(jfkyjf202203001)to G.W.

摘　　要：Nanoparticles(NPs)hold tremendous targeting potential in cardiovascular disease and regenerative medicine,and exciting clinical applications are coming into light.Vascular endothelial cells(ECs)exposure to different magnitudes and patterns of shear stress(SS)generated by blood flow could engulf NPs in the blood.However,an unclear understanding of the role of SS on NP uptake is hindering the progress in improving the targeting of NP therapies.Here,the temporal and spatial distribution of SS in vascular ECs and the effect of different SS on NP uptake in ECs are highlighted.The mechanism of SS affecting NP uptake through regulating the cellular ROS level,endothelial glycocalyx and membrane fluidity is summarized,and the molecules containing clathrin and caveolin in the engulfment process are elucidated.SS targeting NPs are expected to overcome the current bottlenecks and change the field of targeting nanomedicine.This assessment on how SS affects the cell uptake of NPs and the marginalization of NPs in blood vessels could guide future research in cell biology and vascular targeting drugs.

关 键 词：shear stress nanoparticle uptake endothelial cell CLATHRIN CAVEOLIN 

分 类 号：R318[医药卫生—生物医学工程] TB383[医药卫生—基础医学]