CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment  

作　　者：Huifen Qiang Caiyao Hou Yujue Zhang Xin Luo Jun Li Chunxiu Meng Kun Liu Zhaoyong Lv Ximeng Chen Fengzhen Liu 

机构地区：[1]Department of Materials Science and Engineering,Liaocheng University,Liaocheng,China [2]Liaocheng People’s Hospital,Liaocheng Dongchangfu People’s Hospital,Liaocheng 252000,China [3]School of Materials Science and Engineering,University of Science and Technology,Beijing 100083,China

出　　处：《Regenerative Biomaterials》2023年第1期1096-1110,共15页再生生物材料（英文版）

基　　金：supported by the Science Foundation of Shandong Province of China[Grant No.ZR2021MH026,ZR2022MH075];Medicine and Health Science Technology Development plan of Shandong Province of China[Grant No.202108020440,2020Q127];Liaocheng Key Research and Development Plan of Shandong Province of China[Grant No.2022YDSF16,2022YDSF21];Liaocheng People’s Hospital Youth Fund Project[Grant No.LYQN201914].

摘　　要：Immune response is an important factor in determining the fate of bone replacement materials,in which macrophages play an important role.It is a new idea to design biomaterials with immunomodulatory function to reduce inflammation and promote bone integration by regulating macrophages polarization.In this work,the immunomodulatory properties of CaP Zn-Mn-Li alloys and the specific mechanism of action were investigated.We found that the CaP Zn0.8Mn0.1Li alloy promoted the polarization of macrophages toward M2 and reduced inflammation,which could effectively upregulate osteogenesis-related factors and promote new bone formation,indicating the important role of macrophages polarization in biomaterial induction of osteogenesis.In vivo studies further demonstrated that CaP Zn0.8Mn0.1Li alloy could stimulate osteogenesis better than other Zn-Mn-Li alloys implantations by regulating macrophages polarization and reducing inflammation.In addition,transcriptome results showed that CaP Zn0.8Mn0.1Li played an important regulatory role in the life process of macrophages,activating Toll-like receptor signaling pathway,which participated in the activation and attenuation of inflammation,and accelerated bone integration.Thus,by preparing CaP coatings on the surface of Zn-Mn-Li alloys and combining the bioactive ingredient with controlled release,the biomaterial will be imbibed with beneficial immunomodulatory properties that promote bone integration.

关 键 词：Zn-Mn-Li alloys CaP coatings macrophages polarization OSTEOINTEGRATION inflammation 

分 类 号：TG145[一般工业技术—材料科学与工程] R318[金属学及工艺—金属材料]