3D-printed porous functional composite scaffolds with polydopamine decoration for bone regeneration  

作　　者：Jinn Qi Yili Wang Liping Chen Linjie Chen Feng Wen Lijiang Huang Pfukwa Rueben Chunwu Zhang Huaqiong Li 

机构地区：[1]Department of Orthopaedics,Joint Centre of Translational Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325035,P.R.China [2]Joint Centre of Translational Medicine,Zhejiang Engineering Research Center for Tissue Repair Materials,Wenzhou Institute,University of Chinese Academy of Sciences,Wenzhou,Zhejiang 325011,P.R.China [3]University of Chinese Academy of Sciences,Beijing 100049,P.R.China [4]The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,Zhejiang 325035,P.R.China [5]The Affiliated Xiangshan Hospital of Wenzhou Medical University,Ningbo,Zhejiang 315700,P.R.China [6]Department of Chemistry and Polymer Science,Stellenbosch University,Matieland,Stellenbosch 7602,South Africa

出　　处：《Regenerative Biomaterials》2023年第1期1233-1247,共15页再生生物材料（英文版）

基　　金：support from Wenzhou Institute,University of Chinese Academy of Sciences(WIUCASQD2019002,WIUCASZZXF21005);the First Affiliated Hospital of Wenzhou Medical University.

摘　　要：Large size bone defects affect human health and remain a worldwide health problem that needs to be solved immediately.3D printing technology has attracted substantial attention for preparing penetrable multifunctional scaffolds to promote bone reconditioning and regeneration.Inspired by the spongy structure of natural bone,novel porous degradable scaffolds have been printed using polymerization of lactide and caprolactone(PLCL)and bioactive glass 45S5(BG),and polydopamine(PDA)was used to decorate the PLCL/BG scaffolds.The physicochemical properties of the PLCL/BG and PLCL/BG/PDA scaffolds were measured,and their osteogenic and angiogenic effects were characterized through a series of experiments both in vitro and in vivo.The results show that the PLCL/BG2/PDA scaffold possessed a good compression modulus and brilliant hydrophilicity.The proliferation,adhesion and osteogenesis of hBMSCs were improved in the PDA coating groups,which exhibited the best performance.The results of the SD rat cranium defect model indicate that PLCL/BG2/PDA obviously promoted osteointegration,which was further confirmed through immunohistochemical staining.Therefore,PDA decoration and the sustained release of bioactive ions(Ca,Si,P)from BG in the 3D-printed PLCL/BG2/PDA scaffold could improve surface bioactivity and promote better osteogenesis and angiogenesis,which may provide a valuable basis for customized implants in extensive bone defect repair applications.

关 键 词：3D printing bioactive glass composites POLYDOPAMINE ANGIOGENESIS bone regeneration 

分 类 号：R318[医药卫生—生物医学工程] TP391.73[医药卫生—基础医学]