Medium chain length polyhydroxyalkanoates as potential matrix materials for peripheral nerve regeneration  

作　　者：Rinat Nigmatullin Caroline STaylor Pooja Basnett Barbara Lukasiewicz Alexandra Paxinou Lorena RLizarraga-Valderrama John WHaycock Ipsita Roy 

机构地区：[1]Higher Steaks Ltd.,25 Cambridge Science Park Rd,Milton,Cambridge CB40FW,UK [2]School of Life Sciences,College of Liberal Arts and Sciences,University of Westminster,London W1B 2HW,UK [3]Department of Materials Science&and Engineering,The University of Sheffield,Sheffield S37HQ,UK [4]Foundation of Research and Technology Hellas,Institute of Chemical Engineering and High Temperature Chemical Processes(FORTH/ICE-HT),P.O.Box 1414,GR 26504,Rion,Patras,Greece [5]School of Life Sciences,Queen’s Medical Centre,University of Nottingham,Nottingham NG72UH,UK

出　　处：《Regenerative Biomaterials》2023年第1期1248-1260,共13页再生生物材料（英文版）

基　　金：funded by the European Community’s Seventh Framework Programme(FP7-NMP-2013-SME-7)for NEURIMP un-der grant agreement no.604450;CST also acknowledges funding by the Department of Materials Science and Engineering,Faculty of Engineering,University of Sheffield.IR acknowledges funding from the British Council:Innovative and Collaborative Research Partnerships Grants under Pakistan UK Education Gateway(ICRG-2020);Project No:105:006326/DIISB/007/2021;the Engineering and Physical Sciences Research Council(EPSRC)funding for the See More Make More(SM3)project,EP/V012126/1.

摘　　要：Polyhydroxyalkanoates are natural,biodegradable,thermoplastic and sustainable polymers with a huge potential in fabrication of bioresorbable implantable devices for tissue engineering.We describe a comparative evaluation of three medium chain length polyhydroxyalkanoates(mcl-PHAs),namely poly(3-hydroxyoctanoate),poly(3-hydroxyoctanoate-co-3-hydoxydecanoate)and poly(3-hydroxyoctanoate-co-3-hydroxydecanoate-co-3-hydroxydodecanoate),one short chain length polyhydroxyalkanoate,poly(3-hydroxybutyrate),P(3HB)and synthetic aliphatic polyesters(polycaprolactone and polylactide)with a specific focus on nerve regeneration,due to mechanical properties of mcl-PHAs closely matching nerve tissues.In vitro biological studies with NG108-15 neuronal cell and primary Schwann cells did not show a cytotoxic effect of the materials on both cell types.All mcl-PHAs supported cell adhesion and viability.Among the three mcl-PHAs,P(3HO-co-3HD)exhibited superior properties with regards to numbers of cells adhered and viable cells for both cell types,number of neurite extensions from NG108-15 cells,average length of neurite extensions and Schwann cells.Although,similar characteristics were observed for flat P(3HB)surfaces,high rigidity of this biomaterial,and FDA-approved polymers such as PLLA,limits their applications in peripheral nerve regeneration.Therefore,we have designed,synthesized and evaluated these materials for nerve tissue engineering and regenerative medicine,the interaction of mcl-PHAs with neuronal and Schwann cells,identifying mcl-PHAs as excellent materials to enhance nerve regeneration and potentially their clinical application in peripheral nerve repair.

关 键 词：peripheral nerve injury nerve regeneration BIOMATERIALS mcl-polyhydroxyalkanoates Schwann cells NG108-15 

分 类 号：R318[医药卫生—生物医学工程]