A construct of adipose-derived mesenchymal stem cells—laden collagen scaffold for fertility restoration by inhibiting fibrosis in a rat model of endometrial injury  

作　　者：Yangyang Dai Liaobing Xin Sentao Hu Shiqian Xu Dong Huang Xiaoying Jin Jianmin Chen Rachel Wah Shan Chan Ernest Hung Yu Ng William Shu Biu Yeung Lie Ma Songying Zhang 

机构地区：[1]Assisted Reproduction Unit,Department of Obstetrics and Gynecology,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,China [2]Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province,Hangzhou 310016,China [3]MOE Key Laboratory of Macromolecular Synthesis and Functionalization,Department of Polymer Science and Engineering,Zhejiang University,Hangzhou 310027,China [4]Department of Obstetrics and Gynaecology,School of Clinical Medicine,LKS Faculty of Medicine,The University of Hong Kong,Hong Kong SAR 999077,China [5]Shenzhen Key Laboratory of Fertility Regulation,The University of Hong Kong Shenzhen Hospital,Shenzhen 518000,China

出　　处：《Regenerative Biomaterials》2023年第1期1481-1495,共15页再生生物材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(82061160494);the Department of Science and Technology of Zhejiang Province(2023c03033);and the National Health Commission of China(WKJ-ZJ-2301).

摘　　要：Severe endometrium damage causes pathological conditions such as thin endometrium and intrauterine adhesion,resulting in uterine factor infertility.Mesenchymal stem cell(MSC)therapy is a promising strategy in endometrial repair;yet,exogenous MSCs still raise concerns for safety and ethical issues.Human adipose-derived mesenchymal stem cells(ADMSCs)residing in adipose tissue have high translational potentials due to their autologous origin.To harness the high translation potentials of ADMSC in clinical endometrium regeneration,here we constructed an ADMSCs composited porous scaffold(CS/ADMSC)and evaluated its effectiveness on endometrial regeneration in a rat endometrium-injury model.We found that CS/ADMSC intrauterine implantation(i)promoted endometrial thickness and gland number,(ii)enhanced tissue angiogenesis,(iii)reduced fibrosis and(iv)restored fertility.We ascertained the pro-proliferation,pro-angiogenesis,immunomodulating and anti-fibrotic effects of CS/ADMSC in vitro and revealed that the CS/ADMSC influenced extracellular matrix composition and organization by a transcriptomic analysis.Our results demonstrated the effectiveness of CS/ADMSC for endometrial regeneration and provided solid proof for our future clinical study.

关 键 词：adipose-derived mesenchymal stem cells collagen scaffold ENDOMETRIUM FIBROSIS regeneration 

分 类 号：R318.08[医药卫生—生物医学工程]