紫杉醇和丹皮酚在胶质瘤治疗中的联合抗肿瘤作用  

Combined Antitumor Effect of Paclitaxel And Paeonol in the Treatment of Glioma

作　　者：张学娟 贾锋 Zhang Xuejuan;Jia Feng(Yancheng First People's Hospital,Yancheng Jiangsu 224000,China)

机构地区：[1]盐城市第一人民医院,江苏盐城224000

出　　处：《生命科学仪器》2024年第3期40-46,共7页Life Science Instruments

基　　金：江苏省第五期“333”培养资金资助项目,项目编号BRA2020210。

摘　　要：目的探讨分析紫杉醇(Ptx)和丹皮酚(Pae)在胶质瘤治疗中的联合抗肿瘤效果。方法本研究主要采取细胞培养、噻唑蓝(MTT)试验、DAPI染色、细胞流式术等手段,探究通过联合丹皮酚、紫杉醇化疗,能否增强紫杉醇的化疗作用,并减少紫杉醇的化疗剂量,进而减轻其副作用;同时分析Ptx和Pae的协同抗肿瘤作用机制。结果Ptx、Pae两药合用对U87细胞增殖的抑制作用呈剂量依赖性,且不同浓度的Ptx与Pae(125μg/ml)联用时,各浓度组细胞存活率均比单独使用Ptx组显著减少,差异有统计学意义(P<0.05)。表明,Pae协同Ptx促进细胞凋亡可能通过细胞活性氧机制来完成,且Pae协同Ptx可促进细胞ROS的产生及细胞蛋白激酶AKT的磷酸化,并抑制肿瘤的生长。结论Pae能进一步增强Ptx针对胶质瘤细胞的抗肿瘤活性,且Pae联合Ptx主要通过细胞活性氧机制来促进细胞凋亡。Objective:To explore and analyze the combined anti-tumor effect of paclitaxel(Ptx)and paeonol(Pae)in the treatment of glioma.Methods:In this study、cell culture、MTT assay、DAPI staining and flow cytometry were used to explore whether the chemotherapy combined with paeonol and paclitaxel can enhance the chemotherapy effect of paclitaxel and reduce the chemotherapy dose of paclitaxel,thus alleviating its side effects.At the same time,the synergistic anti-tumor mechanism of Ptx and Pae was analyzed.Results:The combination of Ptx and Pae inhibited the proliferation of U87 cells in a dose-dependent manner.When Ptx and PAE were combined with different concentrations(125μg/ml),the cell survival rate of each concentration group was significantly lower than that of Ptx alone,with statistical significance(P<0.05).The results showed that Pae and Ptx could promote apoptosis through the mechanism of reactive oxygen species,and Pae and Ptx could promote ROS production and phosphorylation of protein kinase AKT,and inhibit tumor growth.Conclusion:Pae can further enhance the anti-tumor activity of Ptx against glioma cells,and Pae combined with Ptx can promote apoptosis mainly through the mechanism of reactive oxygen species.

关 键 词：胶质瘤 紫杉醇 丹皮酚 抗肿瘤 噻唑蓝试验、DAPI染色 细胞流式术 

分 类 号：R739.41[医药卫生—肿瘤]