ACLP、COL11A1在胰腺癌组织中的表达及其与临床病理特征和预后的关系  

The expression of ACLP and COL11A1 in pancreatic cancer tissues and their relationship with clinicopathological features and prognosis

作　　者：马仰仰 谢海燕 陈晓泉 张洁 陈洪椿 Ma Yangyang;Xie Haiyan;Chen Xiaoquan;Zhang Jie;Chen Hongchun(Department of Traditional Chinese and Western Medicine,Shaanxi Cancer Hospital,Shaanxi Province,Xi'an 710000,China;不详)

机构地区：[1]陕西省肿瘤医院中西医科,西安710000 [2]陕西中医药大学附属医院肿瘤科,陕西咸阳712000

出　　处：《疑难病杂志》2024年第9期1106-1111,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：陕西省自然科学基础研究计划项目(2021JM-482)。

摘　　要：目的研究胰腺癌中主动脉羧肽酶类样蛋白(ACLP)、Ⅺ型胶原α1(COL11A1)表达及临床预后价值。方法回顾性选择2019年1月—2021年1月陕西省肿瘤医院中西医科和陕西中医药大学附属医院肿瘤科手术治疗的胰腺癌患者88例为研究对象。采用实时荧光定量PCR和免疫组织化学检测癌组织及癌旁组织中ACLP、COL11A1 mRNA表达和蛋白水平;Pearson相关分析ACLP mRNA与COL11A1 mRNA的相关性;Kaplan-Meier法分析ACLP、COL11A1表达对胰腺癌患者生存预后的影响;Cox回归分析胰腺癌预后的影响因素。结果胰腺癌患者癌组织ACLP、COL11A1 mRNA的相对表达量高于癌旁组织(t/P=31.058/<0.001,27.642/<0.001);胰腺癌患者癌组织中ACLP、COL11A1阳性率分别为69.32%(61/88)、70.45%(62/88),高于癌旁组织的9.09%(8/88)、6.82%(6/88),差异有统计学意义(χ^(2)=66.963、75.155,P均<0.001);胰腺癌组织中ACLP mRNA与COL11A1 mRNA表达呈正相关(r=0.642,P<0.001);TNM分期ⅡB~Ⅲ期、有淋巴结转移的胰腺癌组织中ACLP、COL11A1蛋白阳性率高于TNM分期Ⅰ~ⅡA期、无淋巴结转移(ACLP:χ^(2)/P=19.704/<0.001、12.908/<0.001;COL11A1:χ^(2)/P=22.440/<0.001、14.569/<0.001)。ACLP阳性组3年总生存率为22.95%(14/61),低于阴性组44.44%(12/27),差异有统计学意义(Log rankχ^(2)=5.433,P=0.020);COL11A1阳性组3年总生存率为20.97%(13/62),低于阴性组50.00%(13/26),差异有统计学意义(Log rankχ^(2)=7.281,P=0.007)。TNM分期ⅡB~Ⅲ期、淋巴结转移、ACLP阳性、COL11A1阳性是影响胰腺癌患者预后的独立危险因素[HR(95%CI)=1.781(1.199~2.646),1.962(1.172~3.285),1.505(1.066~2.125),1.568(1.117~2.201)]。结论胰腺癌组织中ACLP、COL11A1 mRNA表达和蛋白水平均明显上调,二者在胰腺癌的发生和进展中发挥促进作用,是新的临床上评估胰腺癌患者预后的标志物。Objective To study the expression and clinical prognostic value of aortic carboxypeptidase like protein(ACLP)and collagen type XI alpha 1 chain(COL11A1)in pancreatic cancer.Methods From January 2019 to January 2021,88 patients with pancreatic cancer who underwent surgical treatment in Shaanxi Cancer Hospital and Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine were selected as the study subjects.Real time fluorescence quantitative PCR and immunohistochemistry were used to detect the expression of ACLP and COL11A1 mRNA and protein in cancer tissues and adjacent tissues.Pearson correlation analysis was used to analyze the correlation between ACLP mRNA and COL11A1 mRNA.Kaplan Meier method was used to analyze the effect of ACLP and COL11A1 expression on the survival and prognosis of patients with pancreatic cancer.Cox regression analysis was used to analyze the prognostic factors of pancreatic cancer.Results The relative expression levels of ACLP and COL11A1 mRNA in pancreatic cancer tissues were higher than those in adjacent tissues(t/P=31.058/<0.001,27.642/<0.001);The positive rates of ACLP and COL11A1 in pancreatic cancer tissues were 69.32%(61/88)and 70.45%(62/88),respectively,which were higher than 9.09%(8/88)and 6.82%(6/88)in adjacent tissues(χ^(2)=66.963,75.155,P<0.001);The expression of ACLP mRNA was positively correlated with that of COL11A1 mRNA in pancreatic cancer tissues(r=0.642,P<0.001);The positive rates of ACLP and COL11A1 protein in pancreatic cancer tissues with TNM stage IIB~Ⅲand lymph node metastasis were higher than those in pancreatic cancer tissues with TNM stage I~IIA and no lymph node metastasis(ACLP:χ^(2)/P=19.704/<0.001,12.908/<0.001;COL11A1:χ^(2)/P=22.440/<0.001,14.569/<0.001).The 3-year overall survival rate of ACLP positive group was 22.95%(14/61),which was lower than that of negative group 44.44%(12/27)(Log rankχ^(2)=5.433,P=0.020);The 3-year overall survival rate of COL11A1 positive group was 20.97%(13/62),which was lower than that of negative group 50.00

关 键 词：胰腺癌 主动脉羧肽酶类样蛋白 Ⅺ型胶原α1 预后 

分 类 号：R735.9[医药卫生—肿瘤]