黄花倒水莲总黄酮提取工艺优化与抗氧化活性研究  

The optimum extraction process and antioxidant activity of total flavonoids from Polygala fallax Hemsl.

作　　者：田乾坤 程忠 庞冰瑜 杨定艳 张艳雯[1] TIAN Qiankun;CHENG Zhong;PANG Bingyu;YANG Dingyan;ZHANG Yanwen(Nanning University,Nanning Guangxi 530200,China;China Animal Husbandry Industry Co.,Ltd.,Beijing 100070,China)

机构地区：[1]南宁学院,广西南宁530200 [2]中牧实业股份有限公司,北京100070

出　　处：《中兽医医药杂志》2024年第5期20-26,共7页Journal of Traditional Chinese Veterinary Medicine

基　　金：广西科技基地和人才专项(桂科AD24010031);南宁学院科研基金(2024KY078)。

摘　　要：近年来,临床生产中越来越多的黄酮类活性成分被添加到畜禽饲料中,用于改善产品质量、预防和治疗疾病等,具有广泛的应用前景。探索黄花倒水莲中总黄酮的提取工艺,并将其应用于临床生产是一项非常有前景和意义的研究。本试验基于单因素筛选和响应面优化法,以黄花倒水莲总黄酮提取率为指标,使用超声微波法从黄花倒水莲中提取总黄酮。通过对料液比、乙醇浓度、超声功率、提取时间和超声温度进行单因素试验研究,基于单因素结果差异,选取提取时间、乙醇浓度、料液比3个因素进行响应面工艺优化,确定黄花倒水莲总黄酮的最佳提取工艺,并制得黄花倒水莲总黄酮提取物。此外,采用DPPH·、ABTS+·自由基清除试验对黄花倒水莲总黄酮进行体外抗氧化活性研究。结果表明,黄花倒水莲总黄酮最优提取工艺条件为料液比1∶17(g∶mL)、乙醇浓度70%、提取时间60 min、超声功率225 W、超声温度60℃,最终得到黄花倒水莲总黄酮的提取率为3.63%,与软件预测最优工艺提取率相近,表明该工艺稳定、可行;质量浓度为1.00 g/L的黄花倒水莲总黄酮提取物对DPPH·、ABTS+·自由基清除率最高,分别为72.13%、68.13%,表明黄花倒水莲总黄酮提取物具有良好的体外抗氧化活性。本研究确定了黄花倒水莲总黄酮的最佳提取工艺,并证明其在体外具有较强的抗氧化活性,为黄花倒水莲总黄酮的进一步开发和利用奠定了理论基础。In recent years,more and more flavonoid active ingredients have been added to livestock and poultry feeds in clinical production for improving product quality,preventing and treating diseases,etc.,which has a wide range of application prospects.Exploring the extraction process of total flavonoids from Polygala fallax Hemsl.and applying them to clinical production is a very promising and meaningful research.In this experiment,total flavonoids were extracted from Polygala fallax Hemsl.by ultrasonic microwave method based on one-way screening and response surface optimization method by the extraction rate of total flavonoids from Polygala fallax Hemsl.as an indicator.Single-factor comparative test was conducted to investigate the material-liquid ratio,ethanol concentration,ultrasonic power,ultrasonic time and ultrasonic temperature;based on the difference of single-factor results,three factors,namely,ultrasonic time,ethanol concentration and material-liquid ratio were selected for the response surface process optimization,to determine the optimal extraction process of Polygala fallax Hemsl.total flavonoids and obtain the Polygala fallax Hemsl.total flavonoid extracts;moreover,the in vitro antioxidant activity of Polygala fallax Hemsl.total flavonoid extracts was investigated using DPPH·,ABTS+·free radical scavenging assay.The results showed that the optimal extraction process conditions for Polygala fallax Hemsl.total flavonoids were material-liquid ratio of 1∶17(g∶mL),ethanol concentration of 70%,extraction time of 60 min,ultrasonic power of 225 W,and ultrasonic temperature of 60℃,the final extraction rate of Polygala fallax Hemsl.total flavonoids was 3.63%,which was similar to the software prediction of the optimal process,indicating that the process was stable and feasible.The in vitro antioxidant results showed that Polygala fallax Hemsl.total flavonoids at a concentration of 1.00 g/L had the highest scavenging rate of DPPH·and ABTS+·free radicals,which were 72.13%and 68.13%,respectively,indicating tha

关 键 词：黄花倒水莲 总黄酮 响应面 抗氧化活性 

分 类 号：S853.7[农业科学—临床兽医学]