口腔鳞状细胞癌中SUFU的表达水平及其对细胞生物学行为的影响  

The expression level of SUFU in oral squamous cell carcinoma and its effect on cell biological behavior

作　　者：张林杰 代海涛[2] 王亚 徐江[2] 丁云杉 仵楠 ZHANG Linjie;DAI Haitao;WANG Ya;XU Jiang;DING Yunshan;WU Nan(School of Medicine,Shihezi University,Shihezi 832000,Xinjiang,China;Department of Stomatology,the First Affiliated Hospital of Shihezi University,Shihezi 832000,Xinjiang,China;Department of Stomatology,Bei-jing Shunyi Hospital,Beijing 101300,China)

机构地区：[1]石河子大学医学院,石河子832000 [2]石河子大学第一附属医院口腔科,石河子832000 [3]北京市顺义区医院口腔科,北京101300

出　　处：《医学研究与战创伤救治》2024年第6期561-566,共6页Journal of Medical Research & Combat Trauma Care

基　　金：国家自然科学基金(82160572);石河子大学校级科研项目(ZZZC2022085)。

摘　　要：目的探讨融合抑制因子(SUFU)在口腔鳞状细胞癌(OSCC)中的表达水平及其低表达对OSCC细胞生物学行为的影响。方法利用IHC检测OSCC组织及邻近正常组织中SUFU的蛋白表达,qRT-PCR和Western blot技术测定OSCC细胞(SCC15、CAL27)及人口腔上皮角质细胞(HOK)中SUFU mRNA及蛋白的表达水平,并通过Pearson卡方检验探索其组织蛋白表达与OSCC患者临床病理特征之间的关系;进一步在OSCC细胞株中降低SUFU的表达,通过CCK-8增殖实验、Transwell侵袭实验和划痕愈合实验检测siSUFU组及siNC组细胞的增殖、侵袭和迁移能力。结果实验表明在OSCC组织中SUFU蛋白的表达水平低于邻近正常组织(P<0.05);OSCC细胞中SUFU mRNA及蛋白的表达水平低于人口腔上皮角质细胞(P<0.05);且SUFU蛋白表达与OSCC患者的肿瘤临床分期和分化程度具有相关性(P<0.05);通过转染SUFU干扰片段发现siSUFU组细胞的增殖活性、侵袭细胞数量以及划痕愈合率相比siNC组明显提高(P<0.05)。结论SUFU在OSCC中呈现低表达,与肿瘤的临床分期和分化程度相关,且参与调节OSCC细胞增殖、侵袭和迁移功能,并可能通过调控Hh信号通路影响OSCC的发生及进展。Objective To investigate the expression level of suppressor of fused(SUFU)in oral squamous cell carcinoma(OSCC)and the effect of low expression on the biological behavior of OSCC cells.Methods IHC was used to detect the expression of SUFU protein in OSCC tissues and adjacent normal tissues.The qRT-PCR and Western blot were used to measure the expression lev-els of SUFU mRNA and protein in OSCC cells(SCC15,CAL27)and human oral epithelial keratinocytes(HOK).Meanwhile,Pear-son Chi-square test was used to explore the relationship between histopprotein expression and clinicopathological features of OSCC pa-tients.The expression of SUFU was further reduced in OSCC cell lines,and the proliferation,invasion and migration ability of siSUFU and siNC cells were detected by CCK-8 proliferation assay,Transwell invasion assay and scratch healing assay.Results The expression level of SUFU protein in OSCC tissues was lower than that in adjacent normal tissues(P<0.05).The expression levels of SUFU mRNA and protein in OSCC cells were lower than those in human oral epithelial keratinocytes(P<0.05).The expression of SUFU protein was correlat-ed with the clinical stage and differentiation degree of OSCC patients(P<0.05).By transfecting SUFU interference fragments,it was found that the proliferation activity,the number of invasive cells and the scratch healing rate of siSUFU group were significantly increased compared with siNC group(P<0.05).Conclusion SUFU is low in OSCC,which is related to the clinical stage and differentiation of tumors,and is involved in regulating the proliferation,invasion and migration of OSCC cells,and may affect the occurrence and progression of OSCC by regulating Hh signaling pathway.

关 键 词：口腔鳞状细胞癌 融合抑制因子 HEDGEHOG信号通路 

分 类 号：R739.8[医药卫生—肿瘤]