UV-LED对稻谷表面黄曲霉菌灭活效果研究  

Study on sterilization effect of ultraviolet light emitting diode on paddy surface Aspergillus flavus

作　　者：黄天鸿 金毅 张忠杰 胡科 尹君 HUANG Tianhong;JIN Yi;ZHANG Zhongjie;HU Ke;YIN Jun(School of Food and Strategic Reserves,Henan University of Technology,Zhengzhou 450001,China;Institute of Grain Storage and Logistics,Academy of National Food and Strategic Reserves Administration,Beijing 100037,China;National Engineering Research Centre for Grain Storage and Logistics,Beijing 100037,China;Zhongke Xinhe(Shenzhen)Technology Co.,Ltd,Shenzhen 518000,China)

机构地区：[1]河南工业大学粮食和物资储备学院,河南郑州450001 [2]国家粮食和物资储备局科学研究院粮食储运研究所,北京100037 [3]粮食储运国家工程研究中心,北京100037 [4]中科芯禾(深圳)科技有限公司,广东深圳518000

出　　处：《河南工业大学学报（自然科学版）》2024年第4期126-133,共8页Journal of Henan University of Technology：Natural Science Edition

基　　金：高功率深紫外芯片技术和应用装备联合研发(H23099)。

摘　　要：稻谷储藏期间易发生真菌污染,采用紫外发光二极管(UV-LED)处理以期为稻谷储藏前灭菌处理的优化提供参考依据。以人工接种黄曲霉菌的稻谷为样品,采用UV-LED技术照射稻谷,探究其对稻谷表面黄曲霉菌的杀灭效果,并测定稻谷发芽率和脂肪酸值。结果表明:以照射时间、照射距离、设备功率、稻谷初始带菌量和稻谷水分含量为试验影响因素,稻谷水分含量对杀菌率无显著影响,另外4个因素均对杀菌率有显著影响;在功率10 W、照射距离5 cm、照射时间90 min时,稻谷表面存活的黄曲霉孢子数降低了90%;随着照射时间的增加,稻谷发芽率呈现先上升后下降的趋势,而脂肪酸值没有发生改变。UV-LED处理可以显著抑制稻谷表面黄曲霉菌的生长,且对稻谷储藏品质无明显影响,是一种有效的稻谷储藏前灭菌保质处理的物理技术。Ultraviolet light emitting diode(UV-LED)treatment is a novel non-thermal sterilization technol-ogy.The focus of this study was to investigate the sterilization effect of UV-LED treatment on Aspergillus flavus artificially inoculated on the surface of paddy.Specifically,infected paddy was subjected to the treat-ment device.The number of A.flavus colonies on paddy was counted before and after treatment.Then,the antifungal efficiency of the apparatus against A.flavus on paddy was assessed.Meanwhile,the germi-nation rate and fatty acid value of paddy were assessed to optimize the sterilization treatments for paddy storage.The results demonstrated that UV-LED treatment could significantly inhibit the growth of A.flavus on the surface of paddy.The rate of sterilization was significantly positively correlated with the exposure time,initial bacterial load on the paddy and device power,significantly negatively correlated with the dis-tance from the light source.However,the moisture content of the paddy did not significantly affect the ster-ilization rate.When the treatment was applied at a power of 10 watts,at a distance of 5 cm for 90 minutes,the number of surviving A.flavus spores on the surface of paddy was reduced by 90%.Moreover,as the exposure time increased,the germination rate of the paddy showed an initial increase followed by a decline,while the fatty acid value remained unchanged.In conclusion,the UV-LED treatment shows great promise as a non-thermal sterilization technique.It is an effective physical technology for sterilizing and maintain quality before storage.The germination rate of the paddy,however,is influenced by the duration of the treatment.These findings provide valuable insights for the optimization of sterilization treatments prior to the storage of paddy,thereby potentially improving the quality and safety of stored paddy.

关 键 词：紫外发光二极管 稻谷 杀菌率 黄曲霉菌 

分 类 号：TS207.4[轻工技术与工程—食品科学]