榄绿粗叶木乙酸乙酯提取物通过IL-24/Bcl-2通路诱导肝癌细胞凋亡的机制  

The Mechanism of Hepatocellular Carcinoma Cell Apoptosis Induced by Ethyl Acetate Extract of Oliva Virifolia via IL-24/Bcl-2 Pathway

作　　者：谢永华 温扬敏 XIE Yong-Hua;WEN Yang-Min(Department of Basic Medicine,Quanzhou Medical College,Quanzhou 362011,China)

机构地区：[1]泉州医学高等专科学校基础医学部,福建泉州1362011

出　　处：《中国药物经济学》2024年第8期111-116,125,共7页China Journal of Pharmaceutical Economics

基　　金：福建省中青年教师教育科研项目(JAT201239)。

摘　　要：目的探讨榄绿粗叶木乙酸乙酯提取物(EE-LLA)通过IL-24/Bcl-2通路诱导肝癌细胞凋亡的机制。方法CCK8方法检测EE-LLA对小鼠肝癌H22细胞增殖的影响;流式细胞检测EE-LLA对小鼠肝癌H22细胞的影响;qPCR检测各组细胞中IL-24和Bcl-2基因表达水平;Western blot检测各组细胞中IL-24和Bcl-2蛋白表达水平。结果随着EE-LLA浓度的逐步升高以及作用时间的延长,小鼠肝癌H22细胞的增殖率呈显著下降趋势,与对照组比较差异有统计学意义(P<0.05)。EE-LLA作用H22细胞24 h,细胞半数抑制浓度(IC_(50))为0.91 mg/ml,48 h,IC_(50)为0.72 mg/ml,72 h,IC_(50)为0.75mg/ml。与对照组相比,不同浓度EE-LLA分别作用小鼠肝癌H22细胞48 h,小鼠肝癌H22细胞的IL-24m RNA表达量升高,Bcl-2 mRNA表达量降低,差异有统计学意义(P<0.05),且具有一定的浓度依赖性;与对照组相比,不同浓度(1.2、2.4、4.8 mg/ml)的EE-LLA分别作用48 h后,小鼠肝癌H22细胞的IL-24蛋白表达量增加,Bcl-2蛋白表达量降低,差异有统计学意义(P<0.05)。结论EE-LLA对小鼠肝癌H22细胞增殖具有显著抑制效果,其潜在机制可能涉及上调IL-24基因的表达并伴随Bcl-2基因表达的下调,这一双重调控作用进而触发了肿瘤细胞的凋亡过程。Objective To explore the mechanism of hepatocellular carcinoma cell apoptosis induced by ethyl acetate extract(EE-LLA)through IL-24/Bcl-2 pathway.Methods The effect of EE-LLA on the proliferation of mouse liver cancer H22 cells was detected by CCK8.The effect of EE-LLA on mouse liver cancer H22 cells was detected by flow cytometry.The gene expression levels of IL-24 and Bcl-2 in each group were detected by qPCR.The protein expression levels of IL-24 and Bcl-2 in each group were detected by Western blot.Results As the concentration of EE-LLA gradually increased and the duration of exposure was extended,the proliferation rate of mouse liver cancer H22 cells exhibited a significant downward trend,with a statistically significant difference compared to the control group(P<0.05).The IC_(50)of EE-LLA on H22 cells was 0.91 mg/mlat 24 h,0.72 mg/ml at 48 h and 0.75 mg/ml at 72 h.Compared with the control group,different concentrations of EE-LLA treated mouse hepatocellular carcinoma H22 cells for 48 h,the expression of IL-24 m RNA increased,and the expression of Bcl-2 m RNA decreased,the difference was statistically significant(P<0.05),and there was a certain concentration dependence;the expression of IL-24 protein was increased in H22 cells,while the expression of Bcl-2 protein was decreased,the difference was statistically significant(P<0.05).Conclusion EE-LLA can inhibit the proliferation of mouse liver cancer H22 cells,and its mechanism may be to up-regulate the expression of IL-24 gene and down-regulate the expression of Bcl-2 gene,thereby inducing tumor cell apoptosis.

关 键 词：榄绿粗叶木乙酸乙酯提取物 肝癌 IL-24 BCL-2 

分 类 号：R735.7[医药卫生—肿瘤] R285.5[医药卫生—临床医学]