介质阻挡放电联合脉冲电晕放电灭活水中维氏气单胞菌的效果与机制  

The effect and mechanism of dielectric barrier discharge combined with pulse corona discharge to inactivate of Aeromonas veronii in water

作　　者：祝振飞 燕鹏程 张荣[1] 张敏[1] 屈广周[1] ZHU Zhen-fei;YAN Peng-cheng;ZHANG Rong;ZHANG Min;QU Guang-zhou(College of Natural Resources and Environment,Northwest A&F University,Yangling,Shaanxi Province 712100,China)

机构地区：[1]西北农林科技大学资源环境学院,陕西杨陵712100

出　　处：《中国环境科学》2024年第9期4968-4977,共10页China Environmental Science

基　　金：陕西省自然科学基金资助项目(2021JM-109);陕西省留学人员科技活动项目;中央高校基本科研业务费专项资金资助项目(2452017106)。

摘　　要：将介质阻挡放电(DBD)与脉冲电晕放电(PCD)联合用于水中维氏气单胞菌的灭活,分析了DBD与PCD的协同效应,考察了电压、pH值、载气种类和流量等对DBD联合PCD(DBD/PCD)灭活维氏气单胞菌的影响,探讨了维氏气单胞菌的失活机制,分析了活性物质的种类和作用,并通过斑马鱼攻毒实验验证了DBD/PCD的安全性.结果表明:DBD/PCD对维氏气单胞菌的灭活效果明显高于DBD和PCD单独灭活效果,且二者的协同系数均大于1;经过420s的处理,DBD/PCD可将维氏气单胞菌的浓度降低7个数量级;增加放电电压可显著提高维氏气单胞菌的灭活效率;碱性条件下,氧气作为载气有利于维氏气单胞菌的灭活;在0.5~1.5L/min的范围内,空气流量对灭活效率的影响并不明显.DBD/PCD系统中产生的活性物质首先作用于维氏气单胞菌外膜,使其破裂,然后进入细胞内造成胞内蛋白和毒力基因的损伤,最终导致维氏气单胞菌失活.DBD/PCD系统产生的活性物质·OH、^(1)O_(2)和O_(2)·^(-)在维氏气单胞菌灭活过程中起到了重要作用.斑马鱼攻毒实验表明:DBD/PCD是一种安全高效的水体病原微生物灭活方法.In this study,the DBD combined with PCD (DBD/PCD) was used to inactivate Aeromonas veronii in water.The synergistic effect of DBD and PCD was analyzed,and the effects of voltage,pH,carrier gas type and flow rate on the inactivation efficiency of Aeromonas veronii were investigated.The inactivation mechanism of Aeromonas veronii was explored,The types and effects of active substances were analyzed,and the safety of the DBD/PCD was verified through the experiment of zebrafish challenge.The results showed that the inactivation effect of DBD/PCD to Aeromonas veronii was significantly higher than that of DBD and PCD alone,and their synergistic coefficients were both greater than 1.After 420s of treatment,DBD/PCD can reduce the concentration of Aeromonas veronii by 7 orders of magnitude;Increasing the discharge voltage can significantly improve the inactivation efficiency of Aeromonas veronii;Under alkaline conditions,oxygen as a carrier gas is beneficial for the inactivation of Aeromonas veronii;The effect of air flow rate on inactivation efficiency is not obvious in the range of 0.5~1.5L/min.The active substances produced in the DBD/PCD system first act on the outer membrane of Aeromonas veronii cells,causing them to rupture,and then enter the cells to cause damage to intracellular proteins and the virulence genes,ultimately leading to the inactivation of Aeromonas veronii.Active substances (·OH,^(1)O_(2) and O_(2)^(-)) produced by DBD/PCD system play an important role in the inactivation of Aeromonas veronii.The experiment of zebrafish challenge showed that the DBD/PCD is a safe and efficient method to inactivate pathogenic microorganisms in water bodies.

关 键 词：介质阻挡放电 脉冲电晕放电 维氏气单胞菌 灭活 机制 斑马鱼 

分 类 号：X172[环境科学与工程—环境科学]