具有降解原油和产生生物表面活性剂双功能菌株的特性  

Characterization of a bifunctional strain with crude oil degradation and biosurfactant production

作　　者：李亚君 张宁[1] 张鹏飞[1] 张瑞昌 周鸣[1] 章春芳[2] 魏学锋[1] LI Ya-jun;ZHANG Ning;ZHANG Peng-fei;ZHANG Rui-Chang;ZHOU Ming;ZHANG Chun-fang;WEI Xue-feng(School of Chemistry and Chemical Engineering,Henan University of Science and Technology,Luoyang 471000,China;Ocean College,Zhejiang University,Zhoushan 316021,China)

机构地区：[1]河南科技大学化学化工学院,河南洛阳471000 [2]浙江大学海洋学院,浙江舟山316021

出　　处：《中国环境科学》2024年第9期5293-5302,共10页China Environmental Science

基　　金：国家自然科学基金资助项目(42276150);河南省科技攻关项目(242102321064);河南科技大学大学生创新创业项目(2023185)。

摘　　要：从高盐含油废水中分离出一株产生物表面活性剂的菌株YY-1,通过形态学、生理生化特性和16S r RNA序列分析,该菌属于希瓦氏菌属(Shewanella sp.).Shewanella sp.YY-1分泌生物表面活性剂的临界胶束浓度为700mg/L,表面张力为30.76mN/m,且在较宽的盐度(10~100g/L),温度(25~100℃),pH值(2~10)范围内均表现出良好的化学稳定性.通过薄层色谱、傅立叶红外光谱和气相色谱-质谱分析,生物表面活性剂被鉴定为磷脂类.将菌株Shewanella sp.YY-1应用于原油废水的生物修复中,表现出良好的降解效果,对原油中短链烷烃(C8~C10),中链烷烃(C11~C20),长链烷烃(C21~C44)的去除率可达74.70%,96.68%,99.42%.本研究为生物表面活性剂产生菌在原油废水的生物修复提供优质的菌种来源和理论依据.A biosurfactant (BS)-producing strain YY-1,which was isolated from high-salt oily wastewater,was identified as Shewanella based on morphological,physiological,biochemical characteristics and 16S rRNA gene sequences analysis.The critical micelle concentration of the BS secreted by Shewanella sp.YY-1 was 700mg/L,the surface tension was 30.76mN/m,and the BS showed good chemical stability over a wide range of salinity (10~100g/L),temperature (25~100℃) and pH (2~10).The extracted BS were identified as phospholipids according to thin-layer chromatography,fourier infrared spectroscopy and gas chromatographymass spectrometry.The results of bioremediation of crude oil wastewater with strain Shewanella sp.YY-1 showed favourable degradation performance.The removal efficiency of short-chain alkanes (C8~C10),medium-chain alkanes (C11~C20),and long-chain alkanes (C21~C44) were 74.70%,96.68%,and 99.42%,respectively.This study could provide excellent strain resource and theoretical basis for the bioremediation of crude oil wastewater with BS-producing bacteria.

关 键 词：原油降解 生物表面活性剂 分离 希瓦氏菌 生物修复 

分 类 号：X172[环境科学与工程—环境科学]