比率荧光探针可视化定量检测烟用香精香料中的铅离子  

Visual quantitative determination of lead ions in tobacco flavor samples using a ratiometric fluorescent probe

作　　者：胡立中[1] 黄兰[1] 林辉 林丹 HU Lizhong;HUANG Lan;LIN Hui;LIN Dan(Technical Center,China Tobacco Anhui Industrial Co.,Ltd.,Hefei 230088,China;Institute of Advanced Technology,University of Science and Technology of China,Hefei 230000,China;Hefei Institute of Physical Science,Chinese Academy of Sciences,Hefei 230031,China)

机构地区：[1]安徽中烟工业有限责任公司技术中心,合肥市高新区230088 [2]中国科学技术大学先进技术研究院,合肥市高新区230000 [3]中国科学院合肥物质科学研究院,合肥市230031

出　　处：《烟草科技》2024年第8期11-20,共10页Tobacco Science & Technology

基　　金：安徽中烟工业有限责任公司重大专项项目“烟草及其辅材中重金属的快速可视化定量分析”(2022176)。

摘　　要：为方便、快捷、准确地测量烟用香精香料中的铅离子含量,通过使用蓝色碳量子点作为信号探针,该探针对铅离子产生荧光响应作用,选用一种对铅离子无反应且荧光颜色有所不同的材料作为参比探针,通过调整这两种材料的混合比例,构建比率型荧光探针,建立了对烟用香精香料中铅离子质量分数的可视化定量分析方法。结果表明:①在紫外光照射下,随着铅离子的质量分数从0至20 mg/kg逐步增加,荧光探针溶液的颜色明显从蓝色转变为粉红色。在烟用香精香料中,铅离子的质量分数与探针的荧光强度值比(I_(460)/I_(580))表现出良好的线性关系,且最低检测限为1.32μg/L。②该探针可在5 min内完成对铅离子的检测,并表现出优异的选择性和抗干扰性。③探针溶液的颜色变化可以通过智能手机的红绿蓝色彩模式数值(即RGB值)进行识别,构建数值与铅离子质量分数的对应标准工作曲线,可简化分析过程,实现铅离子的快速检测,检测限为7.04μg/L。④通过对烟用香精香料实际样品的检测,结果的平均回收率在91.14%~103.80%范围内,相对标准偏差在3.01%~5.23%范围内。该方法操作简便、快捷、准确性好,可实现烟用香精香料中铅离子质量分数的现场、快速、可视化定量检测。To find a convenient,rapid and accurate measurement of lead(Pb^(2+))content in tobacco flavors,blue carbon dots(CDs)were used as a signal probe due to their fluorescence response to Pb^(2+).At the same time,a fluorescent material that does not react with Pb^(2+)and exhibits different fluorescence color was used as a reference probe.By adjusting the mixing ratio of the above two materials,a ratiometric fluorescence probe was constructed and used for visual quantitative analysis of Pb^(2+)mass frations in tobacco flavor samples.The results showed that:1)Under ultraviolet irradiation,as the mass fraction of Pb^(2+)increased from 0 to 20 mg/kg,there was a distinct color transition of the probe solution from blue to pink.The mass fraction of Pb^(2+)showed on a good linear relationship with the fluorescence intensity ratio(I_(460)/I_(580))with a limit of detection(LOD)as low as 1.32μg/L.2)The probe demonstrated rapid response capability with detection completed within 5 min,and showed an excellent selectivity and interference resistance.3)The fluorescence color change could be identified by the Red/Green/Blue(RGB value)color mode by smartphones,then a standard working curve corresponding to RGB values and Pb^(2+)mass fraction was established to simplify the analysis process and realize rapid detection of Pb^(2+)with LOD of 7.04μg/L.4)The average recoveries ranged from 91.14%to 103.80%,and the relative standard deviations(RSDs)were from 3.01%to 5.23%.This method is simple,fast and accurate,and realizes the rapid visual quantitative detection of Pb^(2+)mass fraction in tobacco flavor samples.

关 键 词：烟用香精香料 铅离子 碳点 比率荧光探针 可视化定量检测 

分 类 号：TS264.3[轻工技术与工程—发酵工程]