Bacterial microcompartment-mimicking Pickering emulsion droplets for detoxification of chemical threats under sweet conditions  

作　　者：Xiao Xu Wenqi Xie Ting Wu Chen Chen Xiaoning Chen Yuheng Yang Hao Lian Hao Chen Xiaofeng Cheng Zhitong Chen Guojun Chen Chaoqun Liu Lanlan Chen Yu He Zhaowei Chen Bernard P.Binks Huanghao Yang 

机构地区：[1]New Cornerstone Science Laboratory,MOE Key Laboratory for Analytical Science of Food Safety and Biology,College of Chemistry,Fuzhou University,Fuzhou 350002,China [2]Institute of Biomedical and Health Engineering,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences,Shenzhen 518055,China [3]Department of Biomedical Engineering and the Rosalind&Morris Goodman Cancer Research Institute,McGill University,Montreal QC H3A 1A3,Canada [4]School of Pharmacy,Henan University,Kaifeng 475004,China [5]Department of Chemistry,University of Hull,Hull HU67RX,UK

出　　处：《Science China Chemistry》2024年第9期3039-3049,共11页中国科学（化学英文版）

基　　金：supported by the National Key Research and Development Program of China(2020YFA0210800);the National Natural Science Foundation of China(22334004,22027805,22277011,22107019,22176035);the Major Project of Science and Technology of Fujian Province(2020HZ06006)。

摘　　要：Chemical warfare agents represent a severe threat to mankind and their efficient decontamination is a global necessity.However,traditional disposal strategies have limitations,including high energy consumption,use of aggressive reagents and generation of toxic byproducts.Here,inspired by the compartmentalized architecture and detoxification mechanism of bacterial microcompartments,we constructed oil-in-water Pickering emulsion droplets stabilized by hydrogen-bonded organic framework immobilized cascade enzymes for decontaminating mustard gas simulant(2-chloroethyl ethyl sulfide,CEES)under sweet conditions.Two exemplified droplet systems were developed with two-enzyme(glucose oxidase/chloroperoxidase)and threeenzyme(invertase/glucose oxidase/chloroperoxidase)cascades,both achieving over 6-fold enhancement in decontamination efficiency compared with free enzymes and>99% selectivity towards non-toxic sulfoxide.We found that the favored mass transfer of sugars and CEES from their respective phases to approach the cascade enzymes located at the droplet surface and the facilitated substrate channeling between proximally immobilized enzymes were key factors in augmenting the decontamination efficacy.More importantly,the robustness of immobilized enzymes enabled easy reproduction of both the droplet formation and detoxification performance over 10 cycles,following long-term storage and in far-field locations.

关 键 词：Pickering emulsion biomimetics chemical warfare agent bacterial microcompartment BIOCATALYSIS 

分 类 号：TJ92[兵器科学与技术—武器系统与运用工程] TQ426.97[化学工程]