基于3D皮肤模型的牡丹籽油抗光老化功效研究  

Evaluation for Anti-photoaging Efficacy of Peony Seed Oil by 3D Skin Model

作　　者：温艺慈 刘海军 张晔翔 亓丰伟 Wen Yici;Liu Haijun;Zhang Yexiang;Qi Fengwei(Shandong Huawutang Biotechnology Co.,Ltd.,Jinan 250000,China)

机构地区：[1]山东花物堂生物科技有限公司,山东济南250000

出　　处：《广东化工》2024年第15期65-67,共3页Guangdong Chemical Industry

摘　　要：以牡丹籽油为实验材料,利用紫外线(Ultraviolet,UV)照射3D皮肤模型模拟体外光老化环境,通过供试样品牡丹籽油的作用,考察活性氧自由基(Reactive Oxygen Species,ROS)含量、Ⅳ型胶原蛋白(CollagenⅣ)表达量及脱氧核糖核酸(Deoxyribonucleic Acid,DNA)损伤指标环丁烷嘧啶二聚体(Cyclobutanepyrimidinedimmer,CPD)阳性细胞率,并进一步评价牡丹籽油对人体皮肤的抗光老化功效。结果显示,牡丹籽油可显著降低UV照射后皮肤模型ROS含量(p<0.01),提升Collagen IV含量(P<0.01),同时CPD阳性细胞率显著降低(P<0.01)。实验表明,牡丹籽油能显著减少经UV照射后皮肤的ROS含量,提升CollagenⅣ含量,降低CPD阳性细胞率,从而对皮肤起到一定的抗光老化功效。Using peony seed oil as experimental material,UV irradiation was used to simulate the in vitro photoaging environment of 3D skin model,and the anti-photoaging efficacy of peony seed oil was verified by detecting the content of Reactive Oxygen Species,the expression of collagen IV and the positive cell rate of cyclobutanepyrimidinemer,a DNA damage index,through the effect of peony seed oil.The results showed that peony seed oil could significantly reduce the content of ROS(p<0.01)and increase the collagenIV content(P<0.01)in skin after UV irradiation,while the CPD positive cell rate decreased significantly(P<0.01).The experiment shows that peony seed oil can significantly reduce the content of ROS,improve the content of collagenⅣin skin after UV irradiation,and reduce the rate of CPD positive cells,thus playing a certain anti-photoaging effect on skin.

关 键 词：牡丹籽油 3D皮肤模型 抗光老化 活性氧自由基 Ⅳ型胶原蛋白 

分 类 号：TQ[化学工程]