膜分离耦合离子交换层析制备高纯度α-乳白蛋白  

Membrane Separation Followed by Ion Exchange Chromatography for Preparation of High-Purityα-Lactalbumin

作　　者：于淼[1,2] 许晓曦 吕加平[1] 逄晓阳[1] 张书文[1] 王筠钠[1] YU Miao;XU Xiaoxi;LÜJiaping;PANG Xiaoyang;ZHANG Shuwen;WANG Yunna(Institute of Food Science and Technology,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Food Science,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]中国农业科学院农产品加工研究所,北京100193 [2]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《乳业科学与技术》2024年第4期12-18,共7页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：“十四五”国家重点研发计划重点专项(2021YFD2100700);乳业科技创新基金项目(CDIAKCJJ-MN-2023-03);国家现代农业(奶牛)产业技术体系建设专项(CARS-36)。

摘　　要：以生牛乳为原料,经膜分离及喷雾干燥制得鲜乳乳清蛋白粉,结合离子交换层析制备高纯度α-乳白蛋白。结果表明:在浓缩3倍、微滤1次、洗滤2次的膜分离条件下,50 nm陶瓷膜渗透液中α-乳白蛋白占真蛋白的21.04%,高于100 nm陶瓷膜渗透液(15.84%);对50 nm陶瓷膜渗透液进行喷雾干燥,并进行离子交换层析,层析时,在10倍柱体积内,Na Cl溶液浓度由0 mol/L增至0.5 mol/L,α-乳白蛋白与β-乳球蛋白能较好地分离,得到的α-乳白蛋白和β-乳球蛋白纯度分别为98.18%和97.82%。本研究结果可为工业化制备高纯度α-乳白蛋白乳基料提供技术支持。In this study,ion exchange chromatography was employed to prepare high-purityα-lactalbumin from whey protein powder obtained from raw cow’s milk by sequential membrane separation and spray drying.The results showed thatα-lactalbumin constituted 21.04%of the true protein in the permeate obtained using a 50-nm pore-sized ceramic membrane with three-fold concentration,one cycle of microfiltration and two cycles of washing,which was higher than that obtained using a 100-nm pore-sized ceramic membrane(15.84%).The permeate was spray dried and separated by ion exchange chromatography.Good chromatographic separation ofα-lactalbumin andβ-lactoglobulin was achieved with increasing NaCl concentration from 0 to 0.5 mol/L at up to 10 column volumes,and 98.18%pureα-lactalbumin and 97.82%pureβ-lactoglobulin were obtained.The results of this study provide support for the industrial preparation of high-purityα-lactalbumin.

关 键 词：Α-乳白蛋白 Β-乳球蛋白 分离纯化 膜分离 离子交换层析 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]