一起诺如病毒暴发疫情的病原鉴定及全基因组序列特征分析  

Pathogen identification and characteristics of whole genome sequence analysis of a norovirus epidemic

作　　者：孟卫卫[1] 李方[1] 苏静[1] 林羽佳 袁永和 马鑫[1] MENG Weiwei;LI Fang;SU Jing;LIN Yujia;YUAN Yonghe;MA Xin(Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang 830002,China)

机构地区：[1]新疆维吾尔自治区疾病预防控制中心,新疆乌鲁木齐830002

出　　处：《中国国境卫生检疫杂志》2024年第4期345-348,共4页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的对乌鲁木齐市2022年3月一起诺如病毒暴发疫情进行病原学鉴定,基于全基因组序列进行基因分型及分子进化分析。方法采用实时荧光RT-PCR法对送检的11份粪便样本进行诺如病毒核酸检测,采用二代测序技术对检测Ct值小于30的2份样本进行全基因组测序,用Mega 6.06软件对序列进行系统进化分析及主要衣壳蛋白1(major capsid protein1,VP1)区氨基酸序列变异分析。结果11份样本中有7份经实时荧光RT-PCR检测为诺如病毒GII型阳性,2份样本全基因组序列经序列比对,为诺如病毒GII.P7-GII.6型,与2012年阿根廷株MW305613序列一致性最高,为95.79%,VP1区基因属于GII.6a亚型;VP1区氨基酸序列与2015年中国株KU870455相比有4个氨基酸位点改变。结论本次疫情样本中检出诺如病毒GII.P7-GII.6型,应持续开展对该型别诺如病毒的监测和分子特征研究。Objective To identify the pathogen of a norovirus epidemic in March 2022 in Urumqi,to analyze the genotype and molecular characteristics by whole genome sequencing.Methods Real-time RT-PCR was used to screen pathogens in 11 fecal samples collected from the epidemic.Two samples with Ct value less than 30 were sequenced by using next generation sequencing technology for whole genome sequencing.The Mega 6.06 software was used to analyze the phylogenetic evolution and amino acid sequence variation in the major capsid protein 1(VP1)region.Results Among 11 samples,7 samples were identified norovirus GII positive.Two samples were sequenced and identified as norovirus GII.P7-GII.6 through sequence alignment,the sequence was shared the highest homology with the strain MW305613 reported in Argentina in 2012,and VP1 gene in this study belonged to GII.6a group.There were 4 amino acid changes in VP1 region compared to the Chinese strain KU870455 reported in 2015.Conclusion Norovirus GII.P7-GII.6 was detected positive in this epidemic continuous monitoring and molecular characterization research of this type of norovirus should be carried out.

关 键 词：诺如病毒 基因型 全基因组序列 分子特征 

分 类 号：R184[医药卫生—流行病学]