气腹压在宫颈癌手术治疗中对细胞标志物Ki-67和MMP9表达的影响  

Effect of pneumoperitoneum pressure on the expression of cell markers Ki-67 and MMP9 in surgical treatment of cervical cancer

作　　者：李骥 金欣 刘思聪 李桂林 LI Ji;JIN Xin;LIU Si-cong;LI Gui-lin(Department of Gynecology,Xuzhou Clinical School of Xuzhou Medical University;Department of Gynecology,Affiliated Maternity and Child Health Hospital of Xuzhou Medical University,Xuzhou 221000,China)

机构地区：[1]徐州医科大学徐州临床学院妇科 [2]徐州医科大学附属妇幼保健院妇科,江苏徐州221000

出　　处：《哈尔滨医科大学学报》2024年第3期244-248,253,共6页Journal of Harbin Medical University

基　　金：江苏省科技厅社会发展重点项目(BE2019638);徐州市科技创新项目(KC21247);徐州市卫生健康委科技项目(XWKYSL20210131)。

摘　　要：目的探讨气腹压处理在宫颈癌手术治疗中对人子宫颈癌细胞(human cervical cancer cells,Hela)中Ki-67、基质金属蛋白酶9(matrix metalloproteinase 9,MMP9)表达量的影响。方法通过癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库验证MMP9、Ki-67在宫颈癌中的表达量;收集2021年1月~2023年12月徐州医科大学附属妇幼保健院宫颈癌患者的组织标本,按手术方式分为开腹手术组10例与微创手术组30例,通过Western blot实验和免疫组化实验,检测MMP9、Ki-67在Hela细胞中的表达情况;构建宫颈癌裸小鼠移植瘤模型,分未经气腹压处理组8只与气腹压处理组8只,通过免疫组化实验比较未经气腹压处理的肿瘤组织与气腹压处理2 h后的肿瘤组织的MMP9、Ki-67的表达量。结果(1)MMP9、Ki-67在宫颈癌中的高表达,是宫颈癌的潜在性指标;(2)Western blot结果显示,微创手术组宫颈癌组织标本中的MMP9、Ki-67的表达水平较开腹手术组明显增高(P<0.001),免疫组化结果显示微创手术组宫颈癌组织的MMP9以及Ki-67免疫组化染色的阳性率较开腹手术组明显增高(P<0.001);(3)裸小鼠移植瘤模型实验中免疫组化结果显示,给予气腹压的瘤组织较未经气腹压的瘤组织的MMP9、Ki-67的表达量明显增高(P<0.01;P<0.001)。结论MMP9和Ki-67在肿瘤生物学行为中发挥协同作用,其表达水平可以作为宫颈癌进展的潜在生物标记,并有助于优化宫颈癌患者的治疗方案。Objective To explore the effect of pneumoperitoneum pressure treatment on the expression levels of Ki-67 and matrix metalloproteinase 9(MMP9)in human cervical cancer cells(Hela)during surgical treatment of cervical cancer.Methods The expression of MMP9 and Ki-67 in cervical cancer was verified through The Cancer Genome Atlas(TCGA)database;Tissue specimens of cervical cancer patients in Affiliated Maternity and Child Health Care Hospital of Xuzhou Medical Unniversity from 2021 to 2023 were collected and divided into two groups according to surgical methods:including 10 cases of open surgery group and 30 cases of minimally invasive surgery group.Western blot and immunohistochemistry were used to detect the expression of MMP9 and Ki-67 in Hela cells.The cervical cancer xenograft model in nude mice was established and divided into the untreated group and the pneumoperitoneum pressure treatment group and both of the two groups including 8 nude mices.The expression levels of MMP9 and Ki-67 in the tumor tissues without pneumoperitoneum pressure treatment and the tumor tissues treated with pneumoperitoneum pressure for 2 h were compared by immunohistochemistry.Results(1)The high expression of MMP9 and Ki-67 in cervical cancer was a potential indicator of cervical cancer;(2)Western blot results showed that the expression levels of MMP9 and Ki-67 in cervical cancer tissues of minimally invasive surgery were significantly higher than those of open surgery group(P<0.001).The results of immunohistochemistry showed that the positive rates of MMP9 and Ki-67 immunohistochemical staining in cervical cancer tissues of minimally invasive surgery were significantly higher than those of open surgery group(P<0.001);(3)In nude mice xenograft model experiments,the results of immunohistochemistry showed that the positive rates of MMP9 and Ki-67 immunohistochemical staining in the pneumoperitoneum pressure treatment group were significantly higher than the untreated group(P<0.01;P<0.001).Conclusion MMP9 and Ki-67 play a synergistic role i

关 键 词：气腹压 宫颈癌根治术 KI-67 基质金属蛋白酶9 

分 类 号：R737.33[医药卫生—肿瘤]