宫颈上皮内瘤变患者血清中DCLK1、LTBP2的表达及与临床病理特征、术后复发的关系  

The expression of DCLK1 and LTBP2 in the serum of patients with cervical intraepithelial neoplasia and their relationship with clinical pathological characteristics and postoperative recurrence

作　　者：封媛媛 白璐 FENG Yuanyuan;BAI Lu(Department of Gynecology,the Fifth People's Hospital of Datong,Shanxi Datong 037000,China)

机构地区：[1]大同市第五人民医院妇科,山西大同037000

出　　处：《现代肿瘤医学》2024年第18期3513-3518,共6页Journal of Modern Oncology

摘　　要：目的:探讨宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)患者血清双皮质素样激酶1(bicorticoid like kinase 1,DCLK1)、潜在转化生长因子结合蛋白2(latent transforming growth factor binding protein 2,LTBP2)水平与临床病理特征和术后复发的关系。方法:收集整理2019年03月至2022年09月期间于本院确诊的342例CIN患者的相关临床资料并作为CIN组,依据术后复发情况分为未复发组293例和复发组49例;收集351例健康体检者为对照组。采用酶联免疫吸附法检测血清DCLK1、LTBP2水平;采用受试者工作特征(ROC)曲线分析血清DCLK1、LTBP2水平对CIN患者术后复发的预测价值;采用Cox比例风险回归模型筛选CIN复发的影响因素。结果:CIN组血清DCLK1、LTBP2水平高于对照组(P<0.05)。CIN分级Ⅲ级、有高危人乳头瘤病毒(HPV)感染的CIN患者血清DCLK1、LTBP2水平高于CIN分级Ⅰ-Ⅱ级、无高危HPV感染患者(P<0.05)。复发组血清DCLK1、LTBP2水平及CIN分级Ⅲ级、有高危HPV感染的CIN患者比例高于未复发组(P<0.05)。DCLK1、LTBP2单独及联合预测CIN患者术后复发的AUC分别为0.803、0.710、0.870。DCLK1、LTBP2、CIN分级是CIN患者术后复发的独立危险因素(P<0.05)。结论:CIN患者血清DCLK1、LTBP2高表达与CIN分级增加和高危HPV感染有关,且二者联合可有效预测术后复发。Objective:To investigate the relationship between levels of serum bicorticoid like kinase 1(DCLK1)and latent transforming growth factor binding protein 2(LTBP2)in patients with cervical intraepithelial neoplasia(CIN)and their clinical pathological characteristics,postoperative recurrence.Methods:The relevant clinical data of 342 CIN patients diagnosed in our hospital from March 2019 to September 2022 were collected and organized as the CIN group,according to postoperative recurrence,there were 293 cases in the non recurrence group and 49 cases in the recurrence group.351 healthy individuals were used as a reference group.Enzyme linked immunosorbent assay was applied to detect levels of serum DCLK1 and LTBP2.ROC curve was applied to analyze the predictive value of serum DCLK1 and LTBP2 levels for postoperative recurrence in CIN patients.Cox proportional risk regression model was applied to screen for the influencing factors of CIN recurrence.Results:The levels of serum DCLK1 and LTBP2 in the CIN group were higher than those in the reference group(P<0.05).The levels of serum DCLK1 and LTBP2 in CIN patients with CIN grade III and high-risk human papillomavirus(HPV)infection were higher than those in CIN grade I-II and non high-risk HPV infection patients(P<0.05).The levels of serum DCLK1,LTBP2,and the proportions of CIN grade III,and high-risk HPV infection in the recurrent group were higher than those in the non recurrent group(P<0.05).The AUC for predicting postoperative recurrence in CIN patients using DCLK1 and LTBP2 alone and in combination was 0.803,0.710,and 0.870,respectively.DCLK1,LTBP2,and CIN grading were independent risk factors for postoperative recurrence in CIN patients(P<0.05).Conclusion:The high expression of serum DCLK1 and LTBP2 in CIN patients is associated with increased CIN grading and high-risk HPV infection,and the combination of the two can effectively predict postoperative recurrence.

关 键 词：宫颈上皮内瘤变 双皮质素样激酶1 潜在转化生长因子结合蛋白2 临床病理特征 复发 

分 类 号：R737.33[医药卫生—肿瘤]