直肠癌组织中LncRNA TTN-AS1及角鲨烯环氧酶表达与临床病理特征及预后相关性研究  

Correlation between the Expression of LncRNA TTN-AS1 and Squalene Epoxidase in Rectal Cancer Tissue and Clinicopathological Characteristics and Prognosis

作　　者：付超英 黄士月 张云霞 FU Chaoying;HUANG Shiyue;ZHANG Yunxia(Department of Pathology,Longquan Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610100,China;Department of Pathology,Chengdu Xindu District People’s Hospital,Chengdu 610500,China)

机构地区：[1]成都中医药大学附属医院龙泉医院病理科,成都610100 [2]成都市新都区人民医院病理科,成都610500

出　　处：《现代检验医学杂志》2024年第5期12-16,53,共6页Journal of Modern Laboratory Medicine

基　　金：四川省医学会(青年创新)科研课题(N0:Q20038)。

摘　　要：目的研究直肠癌组织中长链非编码RNA(long non-coding RNA,LncRNA)TTN-AS1和角鲨烯环氧酶(squalene epoxidase,SQLE)的表达与临床病理特征及预后相关性。方法选取2018年1月~2020年1月成都中医药大学附属医院诊治的90例直肠癌患者为研究对象。采用荧光定量PCR检测组织LncRNA TTN-AS1表达。采用免疫组织化学法检测组织SQLE表达。分析LncTTN-AS1,SQLE与直肠癌临床病理特征的关系。KM曲线分析LncRNA TTNAS1,SQLE对直肠癌预后的影响。COX回归分析影响直肠癌预后的因素。结果直肠癌组织中LncRNA TTN-AS1(3.12±0.45)相对表达量,SQLE(71.11%)蛋白阳性率均高于癌旁组织(0.91±0.12,8.89%),差异具有统计学意义(t/χ^(2)=45.156,72.593,均P<0.001)。直肠癌组织中LncRNA TTN-AS1与SQLE表达呈正相关(r=0.589,P<0.001)。TNM分期Ⅲ期、淋巴结转移癌组织中Lnc RNA TTN-AS1相对表达量(4.26±0.52,4.10±0.49)、SQLE(88.57%,91.43%)阳性率高于TNM分期Ⅰ~Ⅱ期(2.39±0.40,60.00%)、无淋巴结转移癌组织(2.50±0.42,58.18%),差异具有统计学意义(t/χ^(2)=8.409~19.211,均P<0.05)。LncRNA TTN-AS1高表达组和低表达组三年生存率分别为50.00%(22/44)和86.96%(40/46),差异具有统计学意义(Log-rankχ^(2)=14.205,P=0.001)。SQLE阳性组和阴性组三年生存率分别为64.06%(41/64)和88.46%(23/26),差异具有统计学意义(Log-rankχ^(2)=6.291,P=0.012)。LncRNATTNAS1高表达(HR=2.552,P=0.001)、SQLE阳性(HR=1.754,P=0.004)、TNM分期Ⅲ期(HR=2.797,P=0.011)和淋巴结转移(HR=1.635,P=0.030)是直肠癌预后的独立危险因素。结论直肠癌组织中LncRNA TTN-AS1,SQLE表达升高,与直肠癌肿瘤TNM分期及淋巴结转移有关,是评估直肠癌预后的肿瘤标志物。Objective To study the expression of long non-coding RNA(LncRNA TTN-AS1)TTN-AS1 and squalene epoxidase(SQLE)in rectal cancer,and their correlation with clinicopathological characteristics and prognosis.Methods A total of 90 rectal cancer patients diagnosed and treated in Longquan Hospital of Chengdu University of Traditional Chinese Medicine from January 2018 to January 2020 were selected as the research subjects.Fluorescence quantitative PCR was used to detect the expression of LncRNA TTN-AS1 in tissues.Immunohistochemistry was used to detect the expression of SQLE in tissues.The relationship between LncRNA TTN-AS1,SQLE and clinicopathological characteristics of rectal cancer were compared.K-M curve analysis was used to analyze the impact of LncRNA TTN-AS1 and SQLE on the prognosis of rectal cancer.COX regression analysis was used to analyze factors affecting the prognosis of rectal cancer.Results The relative expression level of Lnc RNA TTN-AS1(3.12±0.45)and the positivity rate of SQLE(71.11%)protein in rectal cancer tissues were higher than those in adjacent tissues(0.91±0.12,8.89%),and the differences were significant(t/χ^(2)=45.156,72.593,all P<0.001).The expression of Lnc RNA TTN-AS1 was positively correlated with SQLE in rectal cancer tissue(r=0.589,P<0.001).The relative expressions of Lnc RNA TTN-AS1(4.26±0.52,4.10±0.49),SQLE(88.57%,91.43%)in tumor TNM stage III and lymph node metastasis were higher than those in tumor TNM stage I~II(2.39±0.40,60.00%)and tissues without lymph node metastasis(2.50±0.42,58.18%),and the differences were significant(t/χ^(2)=8.409~19.211,all P<0.05).The 3-year survival rates of the LncTTN-AS1 high expression group and low expression group were 50.00%(22/44)and 86.96%(40/46),respectively,and the difference between the curves was significant(Log-rankχ^(2)=14.205,P=0.001).The 3-year survival rates of the SQLE positive and negative groups were 64.06%(41/64)and 88.46%(23/26),respectively,and the difference between the curves was significant(Log-rankχ^(2)=6.291,P=0.012).Ln

关 键 词：直肠癌 长链非编码RNA TTN-AS1 角鲨烯环氧酶 临床病理特征 

分 类 号：R735.37[医药卫生—肿瘤] R730.43[医药卫生—临床医学]