HPV E6E7 mRNA检测和DNA倍体定量分析在不同宫颈组织病理学病变的应用  

Application of HPV E6E7 mRNA detection and DNA ploidy quantitative analysis in different cervical histopathological lesions

作　　者：朱丽霞 李海 李沛瑶 张淑琦 徐海锋 王红燕 周静 陈娟 刘琴 ZHU Lixia;LI Hai;LI Peiyao;ZHANG Shuqi;XU Haifeng;WANG Hongyan;ZHOU Jing;CHEN Juan;LIU Qin(Department of Gynecology,Kunshan First People's Hospital Group,Kunshan Maternal and Child Healthcare Hospital,Kunshan 215300,CHINA)

机构地区：[1]昆山市第一人民医院集团昆山市妇幼保健院妇科,江苏215300 [2]昆山市第一人民医院病理科 [3]南京医科大学姑苏学院昆山市第一人民医院 [4]江苏大学附属昆山医院妇产科 [5]昆山市第一人民医院放射科

出　　处：《江苏医药》2024年第8期783-785,共3页Jiangsu Medical Journal

基　　金：江苏省卫生健康委医学科研项目(Z2023001);江苏省卫生健康委医学科研项目(Z2021084);昆山市重点研发计划(社会发展)项目(KSF202101)。

摘　　要：目的探讨HPV E6E7 mRNA检测和DNA倍体定量分析在不同宫颈组织病理学病变的应用。方法HPV E6E7 mRNA检测阳性或DNA倍体定量分析阳性的500例患者行宫颈活检,以病理学作为金标准,比较HPV E6E7 mRNA检测和DNA倍体定量分析在宫颈炎、宫颈低级别鳞状上皮内病变(LSIL)、宫颈高级别鳞状上皮内病变(HSIL)及宫颈癌中的结果,分析单独及联合检测对宫颈HSIL和宫颈癌的诊断效能。结果HPV E6E7 mRNA检测在宫颈LSIL的阳性率高于宫颈炎,在宫颈HSIL和宫颈癌的阳性率高于宫颈LSIL(P<0.05)。DNA倍体定量分析在宫颈LSIL、宫颈HSIL、宫颈癌的阳性率均高于宫颈炎,在宫颈HSIL和宫颈癌的阳性率高于宫颈LSIL(P<0.05)。单独HPV E6E7 mRNA检测或DNA倍体定量分析及二者联合检测诊断宫颈HSIL和宫颈癌的灵敏度为93.18%或70.45%及95.45%,特异度为13.60%或92.32%及12.94%,准确度为20.60%或90.40%及20.20%。结论HPV E6E7 mRNA和DNA倍体定量分析在不同宫颈组织病理学病变中的阳性率存在差异,单独检测和二者联合检测对宫颈HSIL和宫颈癌有一定的诊断效能。Objective To investigate the application of HPV E6E7 mRNA detection and DNA ploidy quantitative analysis in different cervical histopathological lesions.Methods The cervical biopsy was performed in 500 patients with positive HPV E6E7 mRNA detection or positive DNA ploidy quantitative analysis.Taking pathology as the gold standard,the results of HPV E6E7 mRNA detection and DNA ploidy quantitative analysis in cervicitis,cervical low-grade squamous intraepithelial lesions(LSIL),cervical high-grade squamous intraepithelial lesions(HSIL)and cervical cancer were compared.The diagnostic efficiency of HSIL and cervical cancer by single or combined detection was analyzed.Results The positive rate of HPV E6E7 mRNA detection was higher in cervical LSIL than that in cervicitis,which was higher in cervical HSIL and cervical cancer than that in cervical LSIL(P<0.05).The positive rate of DNA ploidy quantitative analysis in cervical LSIL,cervical HSIL and cervical cancer was higher than that in cervicitis,which was higher in cervical HSIL and cervical cancer than that in cervical LSIL(P<0.05).The sensitivity of HPV E6E7 mRNA detection,DNA ploidy quantitative analysis and combined use of the two detections for HSIL and cervical cancer was 93.18%,70.45%and 95.45%,the specificity was 13.60%,92.32%and 12.94%,and the accuracy was 20.60%,90.40%and 20.20%,respectively.Conclusion The positive rate of HPV E6E7 mRNA detection and DNA ploidy quantitative analysis in different cervical histopathological lesions is various.HPV E6E7 mRNA detection,DNA ploidy quantitative analysis and combined use of the two detections have certain diagnostic efficacy for cervical HSIL and cervical cancer.

关 键 词：人乳头状瘤病毒E6E7信使核糖核酸检测 脱氧核糖核酸倍体定量分析 宫颈癌 

分 类 号：R737[医药卫生—肿瘤]