花旗松素磷脂复合物制备优化及其体外模拟消化释放研究  

Preparation,optimization and in vitro simulated digestion release of taxifolin phospholipid complex

作　　者：霍文凯 孙敬蒙 张鑫 孔繁铭 李冬冬 张炜煜[1] HUO Wenkai;SUN Jingmeng;ZHANG Xin;KONG Fanming;LI Dongdong;ZHANG Weiyu(School of Pharmacy,Changchun University of Traditional Chinese Medicine,Changchun 130117,China;Department of Pharmacy,First Hospital of Jilin University,Changchun 130021,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]吉林大学第一医院临床药学部,吉林长春130021

出　　处：《食品与发酵工业》2024年第17期162-168,共7页Food and Fermentation Industries

基　　金：长春中医药大学2022年研究生培养创新示范项目(2022JP06)。

摘　　要：为了提高花旗松素的利用率,该研究制备花旗松素磷脂复合物,采用Box-Behnken响应面试验优化磷脂复合物制备工艺,并进行表征分析验证磷脂复合物的形成;通过平衡溶解度、油水分配系数及模拟消化实验对理化性质和释放度进行研究。结果表明,磷脂复合物最佳制备条件为花旗松素/磷脂质量比1∶3.71、反应溶剂体积12.82 mL、反应时间2.94 h,此条件下的复合率为95.55%。差示扫描量热分析、傅里叶红外光谱、X射线衍射实验表明花旗松素与磷脂间通过氢键作用形成磷脂复合物,且花旗松素由晶型转变为无定形态。磷脂复合物增加了花旗松素在水中的溶解度和水-正辛醇体系中的油水分配系数;体外模拟消化释放实验显示,6 h内磷脂复合物中花旗松素累积释放由48.26%提升至71.86%。磷脂复合物改善了花旗松素的理化性质和体外释放,为花旗松素的高效应用与食品制剂开发提供研究基础。To improve the utilization rate of taxifolin,taxifolin phospholipid complex was prepared.Response surface test was used to optimize the preparation process of the phospholipid complex and the formation of the phospholipid complex was verified by characterization analysis.The physicochemical properties and release were studied by equilibrium solubility,oil-water partition coefficient,and simulated digestion experiment.Results showed that the optimal preparation conditions for phospholipids complex were the mass ratio of taxifolin to phospholipid of 1:3.71,reaction solvent volume of 12.82 mL,reaction time of 2.94 h,and the recombination rate under these conditions was 95.55%.Differential scanning calorimetric analysis,Fourier infrared spectroscopy,and X-ray diffraction experiments showed that taxifolin and phospholipids formed phospholipid complex through hydrogen bonding,and taxifolin changed from crystal form to amorphous form.Phospholipids complex increased the solubility of taxifolin in water and the oil-water partition coefficient in the water-octanol system.In vitro simulated digestion release experiments showed that the cumulative release of taxifolin in the phospholipid complex within 6 h increased from 48.26%to 71.86%.Phospholipids complex improved the physicochemical properties and in vitro release of taxifolin.It provided a research basis for the efficient application of taxifolin and the development of food preparations.

关 键 词：花旗松素 磷脂复合物 响应面 表征 模拟消化释放 

分 类 号：TS201.2[轻工技术与工程—食品科学]