基于生物信息学分析着丝粒蛋白M在肾透明细胞癌中表达及临床意义  

Analysis of Centromere Protein M Expression in Renal Clear Cell Carcinoma Based on Bioinformatics and Its Clinical Significance

作　　者：夏丽丽 曹伟强 郑金花 刘星 XIA Lili;CAO Weiqiang;ZHENG Jinhua(Pathology Department of Dongguan Tungwah Hospital,Dongguan City,Guangdong Province 523110;不详)

机构地区：[1]东莞东华医院病理科,广东省东莞市523110 [2]深圳市福田区第二人民医院急诊科

出　　处：《医学理论与实践》2024年第18期3069-3073,共5页The Journal of Medical Theory and Practice

摘　　要：目的:基于生物信息学探讨着丝粒蛋白M(CENPM)在肾透明细胞癌(KIRC)中的表达及其临床意义。方法:通过SANGERBOX数据库进行CENPM基因的泛癌分析。通过GEPIA和UALCAN数据库分析在KIRC中CENPM的表达与肿瘤分级分期的相关性以及与肿瘤患者预后相关性。通过String数据库分析相关蛋白互作网络。通过TIMER数据库分析CENPM在肾透明细胞癌表达水平与免疫浸润水平关系。结果:CENPM基因在33种肿瘤中明显上调;与正常组织相比,CENPM基因在KIRC中呈高表达;CENPM与临床分期及肿瘤病理分级具有相关性,随着患者病理分级分期恶性程度的增高,CENPM表达水平也增高;KIRC患者预后与CENPM表达水平呈负相关,高表达的CENPM患者预后较差;CENPM与CENPU、CENPK、CENPA等蛋白具有相互作用,可能共同调控KIRC的发生和发展;CENPM的表达水平与B细胞、树突状细胞免疫浸润水平呈正相关,差异有统计学意义(P<0.05)。结论:在KIRC中,CENPM的表达与预后及免疫浸润水平密切相关,是KIRC患者预后不良因素,有望成为肾透明细胞癌一个潜在预后生物标记物及免疫治疗的靶点。Objective:To investigate the expression and clinical significance of centromere protein M(CENPM)in kidney renal clear cell carcinoma(KIRC)based on bioinformatics.Methods:Pan-cancer analysis of CENPM gene was performed by SANGERBOX database.The correlation between the expression of CENPM in KIRC and tumor grade and stage and the prognosis of tumor patients was analyzed by GEPIA and UALCAN database.The protein interaction network was analyzed by String database.The relationship between the expression of CENPM in KIRC and the level of immune cell infiltration was analyzed by Timer database.Results:CENPM gene was significantly up-regulated in 33 kinds of tumors,and CENPM gene was highly expressed in KIRC compared with normal tissues.CENPM was correlated with clinical stage and pathological grade of tumor,the expression level of CENPM increased with the increase of malignant degree of pathological stage,the prognosis of patients with KIRC was negatively correlated with the level of CENPM expression,and the prognosis of patients with high expression of CENPM was poor.CENPM interacts with CENPU,CENPK,CENPA and other proteins,which may jointly regulate the occurrence and development of KIRC,and the expression level of CENPM is positively correlated with the immune infiltration of B cells and dendritic cells,and the difference is statistically significant(P<0.05).Conclusion:In KIRC,the expression of CENPM is closely related to the prognosis and the level of immune infiltration,and is a poor prognostic factor in patients with KIRC.It is expected to become a potential biomarker of prognosis and a target for immunotherapy in KIRC.

关 键 词：肾透明细胞癌 着丝粒蛋白M 预后 

分 类 号：R737.11[医药卫生—肿瘤]