Amylase intrapancreatic infusion delays insulin release during an intravenous glucose tolerance test,proof of acini–islet–acinar interactions  

作　　者：Kateryna Pierzynowska Piotr Wychowański Kamil Zaworski Jarosław Woliński Janine Donaldson Dominika Szkopek Katarzyna Roszkowicz-Ostrowska Agata Kondej Stefan G Pierzynowski 

机构地区：[1]Department of Biology,Lund University,Lund 22362,Sweden [2]Department of Animal Physiology,The Kielanowski Institute of Animal Physiology and Nutrition,Polish Academy of Sciences,Jabłonna 05110,Poland [3]Anara AB,Trelleborg 23132,Sweden [4]Department of Head and Neck and Sensory Organs,Division of Oral Surgery and Implantology,Institute of Clinical Dentistry,Gemelli Foundation for the University Policlinic,Catholic University of the“Sacred Heart”,Rome 00168,Italy [5]Department of Oral Surgery,Medical University of Gdańsk,Gdańsk 80211,Poland [6]Laboratory of Large Animal Models,The Kielanowski Institute of Animal Physiology and Nutrition,Polish Academy of Sciences,Jabłonna 05110,Poland [7]School of Physiology,Faculty of Health Sciences,University of the Witwatersrand(WITS),Johannesburg 2050,South Africa [8]Department of Medical Biology,Institute of Rural Health,Lublin 20090,Poland

出　　处：《World Journal of Experimental Medicine》2024年第3期101-107,共7页世界实验医学杂志

摘　　要：BACKGROUND The possible existence of an acini–islet–acinar(AIA)reflex,involving mutual amylase and insulin interactions,was investigated in the current acute experiment on pigs.AIM To confirm the existence of an AIA reflex and justify the placement of the exocrine and endocrine pancreatic components within the same organ.METHODS The study was performed on six pigs under general anesthesia.An intravenous glucose tolerance test was performed,with a bolus infusion of 50%glucose to the jugular vein,while amylase(5000 U/kg)or vehicle intrapancreatic infusions were administered via the pancreaticoduodenalis cranialis artery during 30 min with a 1 mL/min flow rate.RESULTS The amylase infusion to pancreatic arterial circulation inhibited and delayed the insulin release peak which is usually associated with the highest value of blood glucose and is typically observed at 15 min after glucose infusion,for>1 h.The intrapancreatic infusion of the vehicle(saline)did not have any effect on the time frame of insulin release.Infusion of 1%bovine serum albumin changed the insulin release curve dramatically and prolonged the high range of insulin secretion,far beyond the glucose peak.CONCLUSION Intrapancreatic arterial infusion of amylase interrupted the integrated glucose–insulin interactions.This confirms an AIA reflex and justifies placement of the exocrine and endocrine pancreatic components within the same organ.

关 键 词：AMYLASE Glucose-insulin-amylase interaction Intravenous glucose tolerance test Acini-islet-acinar axis INSULIN 

分 类 号：R58[医药卫生—内分泌]