Alteration of ascending colon mucosal microbiota in patients after cholecystectomy  

作　　者：Miao-Yan Fan Qiao-Li Jiang Meng-Yan Cui Meng-Qi Zhao Jing-Jing Wang Ying-Ying Lu 

机构地区：[1]Department of Gastroenterology,Jiading Branch of Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201803,China [2]Shanghai Key Laboratory of Pancreatic Diseases,Institute of Translational Medicine,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201620,China [3]Department of Gastroenterology,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 201620,China

出　　处：《World Journal of Gastrointestinal Surgery》2024年第8期2436-2450,共15页世界胃肠外科杂志（英文版）（电子版）

基　　金：the Shanghai Natural Science Foundation Project,No.22ZR1453500;and Jiading District Health Commission Scientific Project,No.2023-KY-01.

摘　　要：BACKGROUND Cholecystectomy is a successful treatment option for gallstones,although the inci-dence of colorectal cancer(CRC)has notably increased in post-cholecystectomy(PC)patients.However,it remains uncertain whether the altered mucosal micro-biota in the ascending colon is related.In total,30 PC patients and 28 healthy controls underwent colonoscopies to collect mucosal biopsy samples.PC patients were divided based on their clinical features.Then,16S-rRNA gene sequencing was used to analyze the amplicon,alpha diversity,beta diversity,and composition of the bacterial communities.Addi-tionally,the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States(PICRUSt)database,sourced from the Kyoto Encyclopedia of Genes and Genomes,was used to predict the functional capabilities of the bac-teria.RESULTS PC patients were comparable with healthy controls.However,PC patients older than 60 years had a distinct composition compared to those under 60 years old.Bacteroidetes richness was considerably higher at the phylum level in PC patients.Bacteroides,Parabacteroides,and Bilophila were more abundant in the PC group than in the control group.Furthermore,PC patients exhibited greater enrichment in metabolic pathways,specifically those related to lipopolysaccharide biosynthesis and vancomycin group antibiotic production,than controls.CONCLUSION This study indicated that the mucosal microbiota in PC patients was altered,perhaps offering new perspectives on the treatment possibilities for CRC and diarrhea following cholecystectomy.

关 键 词：Post-cholecystectomy Ascending colon Mucosal Microbiota Colorectal cancer DIARRHEA 

分 类 号：R735.2[医药卫生—肿瘤]