Anti-biofilm and anti-virulence potential of cell free supernatant of Akkermansia muciniphila against Salmonella  

作　　者：Jiaxiu Liu Wenxiu Zhu Huanhuan Liu Xiaomeng Ren Ningbo Qin Xiaodong Xia 

机构地区：[1]National Engineering Research Center of Seafood,School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China

出　　处：《Food Science and Human Wellness》2024年第5期2677-2689,共13页食品科学与人类健康（英文）

基　　金：funded by National Key Research and Development Program of China(2022YFD2100104);Science and Technology Research Program of the Liaoning Department of Education(J2020044);Shaanxi Key Research and Development project(2019SF-259);LiaoNing Revitalization Talents Program(XLYC1807220).

摘　　要：Akkermansia muciniphila is one of the commensals residing within the mammalian gut and co-evolving with the host.Numerous studies have demonstrated the benefits of A.muciniphila in ameliorating metabolic disorders,while little is known about the antimicrobial potential of A.muciniphila against pathogens.Here,we examined the antimicrobial and anti-virulence properties of cell free supernatant(CFS)of A.muciniphila against Salmonella Typhimurium.CFS retarded bacterial growth and inhibited the motility of S.Typhimurium SL1344 and S.Typhimurium 14028.CFS dose-dependently reduced cell hydrophobicity and auto-aggregation of both strains.Also,CFS from A.muciniphila significantly attenuated biofilm formation.Compared with untreated bacteria,CFS-treated bacteria significantly decreased adhesion and invasion to Caco-2 cells,and reduced intracellular survival in macrophages.CFS maintained antimicrobial properties after treatment with high temperatures and various proteases,while it lost its antimicrobial activity after pH neutralization.Gas chromatography-mass spectrometry(GC-MS)confirmed that A.muciniphila produced a certain amount of acetate and propionate,and ultra-high-performance liquid chromatography-mass spectrometry(UHPLCMS)identified other organic acids and metabolites in CFS.In summary,CFS from A.muciniphila exhibited anti-biofilm and anti-virulence properties against Salmonella and could be potentially utilized in the food industry for controlling Salmonella contamination and reducing infection.

关 键 词：Akkermansia muciniphila SALMONELLA BIOFILM MOTILITY Short-chain fatty acid Adhesion Invasion 

分 类 号：TS201.3[轻工技术与工程—食品科学]