Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota  

作　　者：Qing Liu Teng Ma Cuijiao Feng Yalin Li Hao Jin Xuan Shi Lai-Yu Kwok Yan Shi Tingtao Chen Heping Zhang 

机构地区：[1]Affiliated Stomatological Hospital of Nanchang University,Nanchang 330000,China [2]Department of Endodontics,Affiliated Stomatological Hospital of Nanchang University,The Key Laboratory of Oral Biomedicine of Jiangxi Province,Jiangxi Province Clinical Research Center for Oral Diseases,Nanchang 330000,China [3]Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010000,China [4]Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010000,China [5]Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010000,China [6]National Engineering Research Center for Bioengineering Drugs and the Technologies,Institute of Translational Medicine,Nanchang University,Nanchang 330000,China

出　　处：《Food Science and Human Wellness》2024年第5期2690-2702,共13页食品科学与人类健康（英文）

基　　金：supported by the National Natural Science Foundation of China (31720103911);the China Agriculture Research System of MOF and MARA;the Science and Technology Major Projects of Inner Mongolia Autonomous Region (2021ZD0014)

摘　　要：Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria.Thus,oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis.We recruited 9 caries-free individuals,and 89 dental caries subjects(5 dropouts).Eighty-nine patients were randomized into three groups:caries(n=8;no treatment),control(n=40;filling),and postbiotics(n=41;filling and 14-day Probio-Eco®intervention).Salivary samples were collected at 0 day(after filling)and 14 days.Our results showed that the diversity of dental caries oral microbiota was significantly increased compared with healthy subjects,and filling could restore a healthier oral microbiota partially and temporarily.Thepostbiotics intervention keeps a low alpha-diversity.Co-occurrence network analysis showed that a more stable oral microbiota structure after postbiotics intervention.Taxonomic and functional annotation of the microbiota revealed that postbiotics co-treatment significantly:increased the relative abundance of Pseudomonas and P.reactans,decreased the relative abundance of Prevotella shahii,and enriched the energy metabolism-related pathways.BugBase-predicted phenotypes inferred to an oral microbiota with decreased potential pathogenic bacteria and increased oxidative stress-tolerant bacteria after postbiotics intervention.Collectively,it suggested that postbiotics co-treatment could be a promising strategy that restores the oral microecological balance for dental caries.

关 键 词：Postbiotics Oral microbiota Salivary microbiota Dental caries Pacific Biosciences(PacBio)single molecule real-time(SMRT)sequencing Dental filling 

分 类 号：R781.1[医药卫生—口腔医学]