Structural characteristics of phenylboronic acid-modified astaxanthin ester and its effect on DSS-induced ulcerative colitis by blocking reactive oxygen species and maintaining intestinal homeostasis  

作　　者：Xing Qiao Hongyan Li Qun Gao Zhigao Wang Jie Xu Lu Yang Changhu Xue 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]College of Marine Science and Biological Engineering,Qingdao University of Science and Technology,Qingdao 266042,China [3]Laboratory of Marine Drugs and Biological Products,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China

出　　处：《Food Science and Human Wellness》2024年第5期2754-2764,共11页食品科学与人类健康（英文）

基　　金：provided by the National Key R&D Program of China(2018YFC0311206);the Fundamental Research Funds for the Central Universities of China(202012018).

摘　　要：A novel and reactive oxygen species(ROS)responsive astaxanthin phenylboronic acid derivative(AstaDPBA)was constructed by grafting phenylboronic acid(PBA)onto astaxanthin succinate diester(AstaD),and its chemical structure and physicochemical property were identified.AstaD-PBA could effectively improve the ROS quenching ability in the lipopolysaccharide(LPS)-induced RAW264.7 cell inflammation model.Then,the bioactivity of AstaD-PBA was studied by 4 zebrafish ROS-responsive infl ammatory models induced by LPS,copper(Cu^(2+)),high-fat diet,and dextran sodium sulfate(DSS).The results suggest that AstaD-PBA might have high biosafety and the best effect on ulcerative colitis(UC)induced by DSS.Furtherly,AstaDPBA significantly alleviated and treated weight loss and colonic shrinkage,inhibited infl ammatory cytokines,and maintained microbiota homeostasis to improve UC in C57BL/6J mice.Alistipes and Oscillibacter were expected to be considered UC marker fl ora according to the Metastats analysis and Pearson correlation Mantel test(P<0.01)of 16S rRNA gene sequencing data.In conclusion,AstaD-PBA has been promised to be a functional compound to improve UC and maintain intestinal microbiota homeostasis.

关 键 词：Astaxanthin ester Reactive oxygen species-responsive Ulcerative colitis Intestinal microbiota Inflammatory cytokines 

分 类 号：TS201.4[轻工技术与工程—食品科学]