Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder  

作　　者：Jingjing Fang Ruixuan Geng Seong-Gook Kang Kunlun Huang Tao Tong 

机构地区：[1]Key Laboratory of Precision Nutrition and Food Quality,Key Laboratory of Functional Dairy,Ministry of Education,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China [2]Department of Food Engineering and Solar Salt Research Center,Mokpo National University,Muangun 58554,Korea [3]Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),Ministry of Agriculture,Beijing 100083,China [4]Beijing Laboratory for Food Quality and Safety,Beijing 100083,China

出　　处：《Food Science and Human Wellness》2024年第5期2889-2905,共17页食品科学与人类健康（英文）

基　　金：supported by Beijing Natural Science Foundation(7222249).

摘　　要：The effective intervention strategy for autism spectrum disorder(ASD)are currently limited.Herein,we attempted to evaluate the potential of L-proline(Pro),a multifunctional amino acid,in ameliorating autismlike behaviors and clarify the molecular mechanisms involved by using the typical valproic acid(VPA)-induced mouse model of ASD.Pro significantly attenuates repetitive behaviors and social dysfunction in ASD mice.The correlation analysis revealed that the beneficial effects of Pro on autism-like behaviors are related to the modulation of gut microbiota structure and composition.The histological analysis revealed that Pro could reverse the decrease of Nissl-positive cells in the prefrontal cortex(PFC)induced by VPA exposure.RNA sequencing demonstrated that Pro can also alter the PFC transcriptomic profile distinguished by the regulation of genes involved in Parkinson disease,neuroactive ligand-receptor interaction,oxidative phosphorylation,and mitogen activated protein kinase signaling pathway.Overall,dietary Pro supplementation may be a promising intervention strategy for ASD.

关 键 词：L-PROLINE Autism spectrum disorder Gut microbiota Prefrontal cortex Behavior 

分 类 号：TS201.4[轻工技术与工程—食品科学] R749[轻工技术与工程—食品科学与工程]