Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp.bulgaricus in skimmed milk  

作　　者：Yue Yang Wenxia Zhou Lingyu Yang Yilun Chen Dongxiao Sun-Waterhouse Dapeng Li 

机构地区：[1]Key Laboratory of Food Nutrition and Human Health in Universities of Shandong,College of Food Science and Engineering,Shandong Agricultural University,Tai’an 271018,China

出　　处：《Food Science and Human Wellness》2024年第5期3018-3030,共13页食品科学与人类健康（英文）

基　　金：supported by Natural Science Foundation of China(41907357);Natural Science Foundation of Shandong(ZR2019PC048);the Key R&D project of Shandong Province(2021TZXD007).

摘　　要：Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years,though the biodegradation mechanism and derived intermediate products remain unclear.This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide,chlorpyrifos,in milk by Lactobacillus delbrueckii ssp.bulgaricus using gas chromatography-tandem mass spectrometry(GC-MS/MS)combined with transcriptome analysis.After the strain was cultured for 20 h in the presence of chlorpyrifos,differential expressions of 383 genes were detected,including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase,phosphoesterase,diphosphatase,oxidoreductase,dehydratase,as well as membrane transporters.GC-MS/MS analysis revealed the changes of secondary metabolites in L.bulgaricus during milk fermentation due to chlorpyrifos stress.6-Methylhexahydro-2H-azepin-2-one,2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates,along with the proposed pathways,might be involved in chlorpyrifos biodegradation by L.bulgaricus.

关 键 词：Lactobacillus delbrueckii ssp.bulgaricus Biodegradation CHLORPYRIFOS TRANSCRIPTOMICS METABOLOME 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程] TS201.3[轻工技术与工程—食品科学与工程]